http://www.cnr.it/ontology/cnr/individuo/prodotto/ID226157
Physiological formation of fluorescent and conductive protein microfibers in live fibroblasts upon spontaneous uptake of biocompatible fluorophores (Articolo in rivista)
- Type
- Label
- Physiological formation of fluorescent and conductive protein microfibers in live fibroblasts upon spontaneous uptake of biocompatible fluorophores (Articolo in rivista) (literal)
- Anno
- 2013-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1039/C3IB40064F (literal)
- Alternative label
Ilenia Viola, Ilaria Elena Palamà, Addolorata Maria Luce Coluccia, Mariano Biasiucci, Barbara Dozza, Enrico Lucarelli, Francesca Di Maria, Giovanna Barbarella and Giuseppe Gigli (2013)
Physiological formation of fluorescent and conductive protein microfibers in live fibroblasts upon spontaneous uptake of biocompatible fluorophores
in Integrative biology (Camb., Online)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Ilenia Viola, Ilaria Elena Palamà, Addolorata Maria Luce Coluccia, Mariano Biasiucci, Barbara Dozza, Enrico Lucarelli, Francesca Di Maria, Giovanna Barbarella and Giuseppe Gigli (literal)
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- National Nanotechnology Laboratory, Institute Nanoscience CNR (NNL, CNR-NANO) via
Arnesano, Lecce, Italy;
Dept. of Physics, Sapienza University, p.le A. Moro 2, Rome, Italy;
Hematology and Clinical Proteomics Unit, \"Vito Fazzi\" Hospital, University of Salento, Piazzale
Muratore, 73100 Lecce, Italy;
Italian Institute of Technology (IIT) - Center for Life Nanoscience @ Sapienza, Rome, Italy;
Bone Regeneration Laboratory, Rizzoli Orthopaedic Institute, via di Barbiano 1/10, 40138
Bologna, Italy;
Laboratorio MIST.E-R and gISOF, Consiglio Nazionale Ricerche, Via P. Gobetti 101, 40129
Bologna, Italy
Mediteknology srl., via Arnesano, Lecce, Italy
Dept. Matematica e Fisica 'Ennio De Giorgi',via Monteroni, University of Salento;
Italian Institute of Technology (IIT) -Center for Biomolecular Nanotechnologies, via Arnesano,
Lecce, Italy (literal)
- Titolo
- Physiological formation of fluorescent and conductive protein microfibers in live fibroblasts upon spontaneous uptake of biocompatible fluorophores (literal)
- Abstract
- We have recently reported initial results concerning an original approach to introduce additional properties into fibrillar proteins produced by live fibroblasts and extruded into the ECM. The key to such an approach were biocompatible fluorescent and semiconducting synthetic molecules which penetrated spontaneously the cells and were progressively encompassed via non-bonding interactions during the self-assembly process of the proteins, without altering cell viability and reproducibility. In this paper we demonstrate that the intracellular secretion of fluorescent microfibers can be generalized to living primary and immortalized human/mouse fibroblasts. By means of real-time single-cell confocal microscopy we show that the fluorescent microfibers, most of which display helical morphology, are generated by intracellular coding of the synthetic molecules. We also describe co-localization experiments on the fluorescent microfibers isolated from the cell milieu demonstrating that they are mainly made of type-I collagen. Finally, we report experimental data indicating that the embedded synthetic molecules cause the proteins not only to be fluorescent but also capable of electrical conductivity. (literal)
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