http://www.cnr.it/ontology/cnr/individuo/prodotto/ID226019
Use of Hydroxyacetophenones as Matrices for the Analysis of High Molecular Weight Glutenin Mixtures by Matrix-assisted Laser Desorption/ Ionization Mass Spectrometry (Articolo in rivista)
- Type
- Label
- Use of Hydroxyacetophenones as Matrices for the Analysis of High Molecular Weight Glutenin Mixtures by Matrix-assisted Laser Desorption/ Ionization Mass Spectrometry (Articolo in rivista) (literal)
- Anno
- 1999-01-01T00:00:00+01:00 (literal)
- Alternative label
Domenico Garozzo1*, Rosaria Cozzolino2, Sabrina Di Giorgi2, Salvatore Fisichella2 and
Domenico Lafiandra3 (1999)
Use of Hydroxyacetophenones as Matrices for the Analysis of High Molecular Weight Glutenin Mixtures by Matrix-assisted Laser Desorption/ Ionization Mass Spectrometry
in RCM. Rapid communications in mass spectrometry
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Domenico Garozzo1*, Rosaria Cozzolino2, Sabrina Di Giorgi2, Salvatore Fisichella2 and
Domenico Lafiandra3 (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- 1CNR Institute for Chemistry and Technology of Polymeric Materials, 6 V.le A. Doria, 95125 Catania, Italy
2Department of Chemical Sciences, 6 V.le A. Doria, 95125 Catania, Italy
3Department of Agrobiology and Agrochemistry, University of Tuscia, 01100 Viterbo, Italy (literal)
- Titolo
- Use of Hydroxyacetophenones as Matrices for the Analysis of High Molecular Weight Glutenin Mixtures by Matrix-assisted Laser Desorption/ Ionization Mass Spectrometry (literal)
- Abstract
- A selection of hydroxyacetophenones has been investigated as matrices for the analysis, by matrix-assisted
laser desorption/ionization mass spectrometry (MALDI-MS), of high molecular weight (HMW) glutenin
mixtures from three wheat varieties. Mass spectra were obtained directly from the HMW glutenin extracts
without any preliminary purification and separation steps. According to the quality of the mass spectra,
obtained using different hydroxyacetophenones, it has been possible to classify the matrices on the basis of
their suitability for the analyte properties. Only two of the matrices considered showed to be compatible
with the HMW glutenin mixtures analysis, although a large amount of other highly complex protein
mixtures are present. This study indicates that MALDI-MS, as a stand-alone technique, is particularly
useful for the direct determination of the complete HMW subunits profile and their molecular weights. (literal)
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- Autore CNR
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