Use of Hydroxyacetophenones as Matrices for the Analysis of High Molecular Weight Glutenin Mixtures by Matrix-assisted Laser Desorption/ Ionization Mass Spectrometry (Articolo in rivista)

Type
Label
  • Use of Hydroxyacetophenones as Matrices for the Analysis of High Molecular Weight Glutenin Mixtures by Matrix-assisted Laser Desorption/ Ionization Mass Spectrometry (Articolo in rivista) (literal)
Anno
  • 1999-01-01T00:00:00+01:00 (literal)
Alternative label
  • Domenico Garozzo1*, Rosaria Cozzolino2, Sabrina Di Giorgi2, Salvatore Fisichella2 and Domenico Lafiandra3 (1999)
    Use of Hydroxyacetophenones as Matrices for the Analysis of High Molecular Weight Glutenin Mixtures by Matrix-assisted Laser Desorption/ Ionization Mass Spectrometry
    in RCM. Rapid communications in mass spectrometry
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Domenico Garozzo1*, Rosaria Cozzolino2, Sabrina Di Giorgi2, Salvatore Fisichella2 and Domenico Lafiandra3 (literal)
Pagina inizio
  • 2084 (literal)
Pagina fine
  • 2089 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 13 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1CNR Institute for Chemistry and Technology of Polymeric Materials, 6 V.le A. Doria, 95125 Catania, Italy 2Department of Chemical Sciences, 6 V.le A. Doria, 95125 Catania, Italy 3Department of Agrobiology and Agrochemistry, University of Tuscia, 01100 Viterbo, Italy (literal)
Titolo
  • Use of Hydroxyacetophenones as Matrices for the Analysis of High Molecular Weight Glutenin Mixtures by Matrix-assisted Laser Desorption/ Ionization Mass Spectrometry (literal)
Abstract
  • A selection of hydroxyacetophenones has been investigated as matrices for the analysis, by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), of high molecular weight (HMW) glutenin mixtures from three wheat varieties. Mass spectra were obtained directly from the HMW glutenin extracts without any preliminary purification and separation steps. According to the quality of the mass spectra, obtained using different hydroxyacetophenones, it has been possible to classify the matrices on the basis of their suitability for the analyte properties. Only two of the matrices considered showed to be compatible with the HMW glutenin mixtures analysis, although a large amount of other highly complex protein mixtures are present. This study indicates that MALDI-MS, as a stand-alone technique, is particularly useful for the direct determination of the complete HMW subunits profile and their molecular weights. (literal)
Prodotto di
Autore CNR

Incoming links:


Prodotto
Autore CNR di
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
data.CNR.it