Biologically active cymbidium ringspot virus satellite RNA in transgenic plants suppresses accumulation of DI RNA (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Biologically active cymbidium ringspot virus satellite RNA in transgenic plants suppresses accumulation of DI RNA (Articolo in rivista) (literal)

Anno

• 1992-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/0042-6822(92)90496-C (literal)

Alternative label

• Rubino, L., Carrington, J.C., Russo, M. (1992)
  Biologically active cymbidium ringspot virus satellite RNA in transgenic plants suppresses accumulation of DI RNA
  in Virology (N.Y.N.Y., Print); Academic Press Elsevier, Inc., New York (Stati Uniti d'America)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Rubino, L., Carrington, J.C., Russo, M. (literal)

Pagina inizio

• 429 (literal)

Pagina fine

• 437 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 188 (literal)

Rivista

• Virology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 9 (literal)

Note

• PubMe (literal)
• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• RL,RM: Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee, Bari, Italy CJC: Department of Biology, Texas A&M University, College Station, Texas, USA (literal)

Titolo

• Biologically active cymbidium ringspot virus satellite RNA in transgenic plants suppresses accumulation of DI RNA (literal)

Abstract

• A full-length DNA copy of cymbidium ringspot virus (CyRSV) satellite RNA was cloned downstream of the bacteriophage T7 RNA polymerase promoter. In vitro transcripts were biologically active in plants when coinoculated with the helper virus or its RNA. Although the transcripts contained 7 or 29 extra nucleotides at the 3' end, the proper 3' terminus was restored in the satRNA progeny. Full-length cDNA clones of CyRSV satRNA under the control of the cauliflower mosaic virus 35S promoter and terminator were used to transform Nicotiana benthamiana plants. Integration of CyRSV satRNA sequence in the plant genome was tested by PCR amplification of DNA extracts from transformed plants and by detection of satRNA-related transcripts in total RNA extracts. Inoculation of transgenic plants with the helper virus induced replication of satRNA of the same size as the native molecule. Sequence analysis of the satRNA progeny showed that it was identical to natural CyRSV satRNA. Infected transgenic plants were not protected from apical necrosis and death by the presence of satRNA sequences. Rather, replication of satRNA was found to suppress accumulation of defective interfering RNA, which acts in the absence of satRNA as an attenuator of virus replication and disease. (literal)

Editore

• Academic Press Elsevier, Inc. (Editore)

Prodotto di

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Autore CNR

• LUISA RUBINO (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Biologically active cymbidium ringspot virus satellite RNA in transgenic plants suppresses accumulation of DI RNA" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• LUISA RUBINO (Unità di personale interno)

Prodotto

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Virology (Rivista)

Editore di

• Academic Press Elsevier, Inc. (Editore)

Insieme di parole chiave di

• Keywords of "Biologically active cymbidium ringspot virus satellite RNA in transgenic plants suppresses accumulation of DI RNA" (Insieme di parole chiave)