http://www.cnr.it/ontology/cnr/individuo/prodotto/ID223170
The replication of cymbidium ringspot tombusvirus defective interfering-satellite RNA hybrid molecules (Articolo in rivista)
- Type
- Label
- The replication of cymbidium ringspot tombusvirus defective interfering-satellite RNA hybrid molecules (Articolo in rivista) (literal)
- Anno
- 1992-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/0042-6822(92)90895-V (literal)
- Alternative label
Burgyan, J., Dalmay, T., Rubino, L., Russo, M. (1992)
The replication of cymbidium ringspot tombusvirus defective interfering-satellite RNA hybrid molecules
in Virology (N.Y.N.Y., Print); Academic Press Elsevier, Inc., New York (Stati Uniti d'America)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Burgyan, J., Dalmay, T., Rubino, L., Russo, M. (literal)
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- Note
- ISI Web of Science (WOS) (literal)
- PubMe (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- BJ, DT: Agricultural Research Center, PO Box 170, H-2101 Gödöllö, Hungary
RL, RM: Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee, Via Amendola 165/A, 70126 Bari, Italy (literal)
- Titolo
- The replication of cymbidium ringspot tombusvirus defective interfering-satellite RNA hybrid molecules (literal)
- Abstract
- A DNA copy of DI RNA of cymbidium ringspot tombusvirus was cloned downstream of a phage T7 promoter. In vitro-transcribed RNA replicated in Nicotiana clevelandii when co-inoculated with full-length viral genomic RNA transcripts and protected plants from apical necrosis. Artificial deletion mutants derived from the DI RNA clone showed that most of the central sequence block is necessary for replication. Hybrid DI RNA-satRNA clones were prepared and in vitro-synthesized RNA was inoculated to plants in the presence of helper viral RNA. There was replication only of in vitro transcripts derived from hybrid clones where satRNA sequences were inserted upstream or downstream from the central block, but not of those derived from clones where satRNA sequence replaced the central block. Progeny RNA of biologically active clones was either full-length or showed deletions depending on the insertion of satRNA sequences in DI RNA. DI RNA-satRNA constructs having part of the 5' region exchanged were not replicated. (literal)
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