http://www.cnr.it/ontology/cnr/individuo/prodotto/ID222416

De novo generation of cymbidium ringspot virus defective interfering RNA (Articolo in rivista)

Type

Label

De novo generation of cymbidium ringspot virus defective interfering RNA (Articolo in rivista) (literal)

Anno

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

Alternative label

BURGYAN, J., RUBINO, L., RUSSO, M. (1991)
De novo generation of cymbidium ringspot virus defective interfering RNA
in Journal of general virology (Print); Society for general microbiology, Reading (Regno Unito)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Pagina inizio

Pagina fine

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

Rivista

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

Note

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

JB: Agricultural Research Center, PO Box 170, H-2101 Gödöllö, Hungary LR, MR: Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee, Via Amendola 165/A, 70126 Bari, Italy (literal)

Titolo

De novo generation of cymbidium ringspot virus defective interfering RNA (literal)

Abstract

Nicotiana clevelandii plants were inoculated with cymbidium ringspot tombusvirus RNA synthesized in vitro, after which further passages were made by sap inoculation. During the third passage, low Mr RNA species appeared which had the characteristics of deletion mutants of genomic RNA. Sequence analysis of several of these defective interfering RNAs suggested a possible evolution of smaller from larger molecules. Computer-generated secondary structures of sequences surrounding recombination sites were extensive and stable and these sites occurred in interior or hairpin loops, thus providing a possible explanation for discontinuous RNA transcription and the formation of deletions in genomic RNA. (literal)

Editore