http://www.cnr.it/ontology/cnr/individuo/prodotto/ID222299

Characterisation of ALS genes in the polyploid species Schoenoplectus mucronatus and implications for resistance management (Articolo in rivista)

Type

Prodotto della ricerca (Classe)
Articolo in rivista (Classe)

Label

Characterisation of ALS genes in the polyploid species Schoenoplectus mucronatus and implications for resistance management (Articolo in rivista) (literal)

Anno

2009-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1002/ps.1883 (literal)

Alternative label

Laura Scarabel, Antonella Locascio, Antonella Furini, Maurizio Sattin, Serena Varotto (2009)
Characterisation of ALS genes in the polyploid species Schoenoplectus mucronatus and implications for resistance management
in Pest management science (Online)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Laura Scarabel, Antonella Locascio, Antonella Furini, Maurizio Sattin, Serena Varotto (literal)

Pagina inizio

337 (literal)

Pagina fine

344 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

http://onlinelibrary.wiley.com/doi/10.1002/ps.1883/abstract (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

66 (literal)

Rivista

Pest management science (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

8 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

3 (literal)

Note

Google Scholar (literal)
ISI Web of Science (WOS) (literal)
Scopus (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

Istituto di Biologia Agroambientale e Forestale-CNR, Agripolis, Legnaro (PD), Italy Dipartimento di Agronomia Ambientale e Produzioni Vegetali, Università di Padova, Agripolis, Legnaro (PD), Italy Dipartimento Scientifico e Tecnologico, Università di Verona, Verona, Italy (literal)

Titolo

Characterisation of ALS genes in the polyploid species Schoenoplectus mucronatus and implications for resistance management (literal)

Abstract

BACKGROUND: The polyploid weed Schoenoplectus mucronatus (L.) Palla has evolved target-site resistance to ALS-inhibiting herbicides in Italian rice crops. Molecular and genetic characterisation of the resistance mechanism is relevant to the evolution and management of herbicide resistance. The authors aimed (a) to study the organisation of the target-site loci in two field-selected S. mucronatus populations with different cross-resistance patterns, (b) to identify the mutations endowing resistance to ALS inhibitors and determine the role of these mutations by using transgenesis and (c) to analyse the implications for the management of the S. mucronatus populations. RESULTS: Two complete ALS genes (ALS1 and ALS2) having an intron and a third partial intronless ALS gene (ALS3) were identified. The presence of multiple ALS genes was confirmed by Southern blot analyses, and ALS loci were characterised by examining cytosine methylation. In S. mucronatus leaves, the transcripts of ALS1, ALS2 and ALS3 were detected. Two mutations endowing resistance (Pro197 to His and Trp574 to Leu) were found in both resistant populations, but at different frequencies. Tobacco plants transformed with the two resistant alleles indicated that the Pro197-to-His substitution conferred resistance to SU and TP herbicides, while the allele with the Trp574-to-Leu substitution conferred cross-resistance to SU, TP, IMI and PTB herbicides. CONCLUSION:Schoenoplectus mucronatus has multiple ALS genes characterised by methylated sites that can influence the expression profile. The two mutated alleles proved to be responsible for ALS resistance. At population level, the resistance pattern depends on the frequency of various resistant genotypes, and this influences the efficacy of various ALS-inhibiting herbicides. Copyright © 2009 Society of Chemical Industry (literal)

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