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Properties of a new strain of tobacco streak virus from Clematis vitalba (Ranunculaceae) (Articolo in rivista)

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Properties of a new strain of tobacco streak virus from Clematis vitalba (Ranunculaceae) (Articolo in rivista) (literal)

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Alternative label

RANA, G.L., KRAJACIC, M., STEFANAC, Z., PLESE, N., RUBINO, L., MILICIC, D. (1987)
Properties of a new strain of tobacco streak virus from Clematis vitalba (Ranunculaceae)
in Annals of Applied Biology; Wiley-Blackwell, Oxford (Regno Unito)
(literal)

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RANA, G.L., KRAJACIC, M., STEFANAC, Z., PLESE, N., RUBINO, L., MILICIC, D. (literal)

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GLR: Istituto di Patologia Vegetale e Forestale, Università della Basilicata, 85100 Potenza, Italy MK, ZS, NP, DM: Department of Biology, Faculty of Science, University of Zagreb, 41000 Zagreb, Yugoslavia LR: Dipartimento di Patologia Vegetale, Università degli Studi, Bari, Italy (literal)

Titolo

Properties of a new strain of tobacco streak virus from Clematis vitalba (Ranunculaceae) (literal)

Abstract

A distinct strain of tobacco streak virus (TS V/Cle), isolated in Yugoslavia from wild Clematis vitalba showing chlorotic spots or yellow netting of the leaves and from many symptomless shrubs, is described. TSV/Cle was seed transmitted in C. vitalba (70%), and in the experimental hosts Chenopodium quinoa (80%), Nicotiana benthamiana and N. megalosiphon. It was also detected in the pollen of infected C. quinoa. Purified virus preparations mostly contained quasi-spherical particles measuring 24-26 × 28, 26-28 × 28-30 and 28-31 × 32-36 mn, and sedimented in sucrose density gradient and analytical centrifugation as three components with sedimentation coefficients of 76S, 87S and 98S. The virus contained a single polypeptide species of mol. wt of c. 25 000. Unfractionated TSV/Cle preparations contained four RNA species with mol. wts, estimated by gel electrophoresis in agarose, of 1.1 × 106, 0.9 × 106, 0.7 × 106 and 0.3 × 106. In comparative experiments, TSV/Cle differed from four reference strains of TSV (TSV/B, TSV/HF, TSV/RN, and TSV/Ro) in host range and in symptoms induced in some common hosts. In agar gel double diffusion tests it was more closely related to TSV/B and TSV/M (SDI = 5) than to TSV/HF (SDI = 7), TSV/RN (SDI = 7) or TSV/Ro (SDI = 5-8). Immunoelectrophoresis experiments clearly distinguished TSV/Cle from the reference strains. TSV/Cle strain was detected in C. vitalba plants from distant and climatically different regions in Yugoslavia. (literal)

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