Shotgun proteomics and network analysis of neuroblastoma cell lines treated with curcumin (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Shotgun proteomics and network analysis of neuroblastoma cell lines treated with curcumin (Articolo in rivista) (literal)

Anno

• 2012-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1039/c2mb05498a (literal)

Alternative label

• Simona D'Aguanno a,b, Igea D'Agnano c, Michele De Canio a, Claudia Rossi d,e, Sergio Bernardini a, Giorgio Federici a and Andrea Urbani a,b (2012)
  Shotgun proteomics and network analysis of neuroblastoma cell lines treated with curcumin
  in Molecular bioSystems (Online)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Simona D'Aguanno a,b, Igea D'Agnano c, Michele De Canio a, Claudia Rossi d,e, Sergio Bernardini a, Giorgio Federici a and Andrea Urbani a,b (literal)

Pagina inizio

• 1068 (literal)

Pagina fine

• 1077 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 8 (literal)

Rivista

• Molecular bioSystems (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 10 (literal)

Note

• PubMe (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• a Department of Internal Medicine, University of Rome Tor Vergata, b Laboratory of Proteomics and Metabonomics, S. Lucia Foundation--IRCCS, Rome, Italy c CNR-Institute of Cell Biology and Neurobiology, Rome, Italy d Centre of Study on Aging (Ce.S.I.), ''G. d'Annunzio'' University Foundation, Chieti, Italy e Department of Biomedical Science, ''G. d'Annunzio University'', Chieti-Pescara, Italy (literal)

Titolo

• Shotgun proteomics and network analysis of neuroblastoma cell lines treated with curcumin (literal)

Abstract

• Curcumin is a natural compound with recognized anti-inflammatory properties, but its anticancer activity is still object of study. We provided an unsupervised molecular investigation of the main proteome rearrangements involved in the cellular response to curcumin in a human neuroblastoma cell line sensitive to cisplatin and its resistant counterpart by a comparative proteomic approach. Shotgun analysis demonstrated that 66 proteins were differentially expressed in response to 24 h treatment with 40 mM curcumin in sensitive cells, whereas 32 proteins were significantly modulated in treated resistant cells. Functional analysis revealed that proteins involved in cellular assembly and organization, biosynthesis and glycolysis were down-regulated by curcumin treatment. Proteome changes were associated to cell cycle arrest in the G2/M phase and accumulation of polyubiquitinated proteins, also confirmed by flow cytometry and immunoblotting analysis, but not to a significant increment of reactive oxygen species production. Since the polyubiquitination of proteins influences a wide range of cellular pathways, the inhibition of the ubiquitin-proteasome system may be the main way through which curcumin performs its multi-target activity. (literal)

Prodotto di

• ex ME.P03.007.001 / Meccanismi regolativi del Differenziamento e Oncogenesi (ME.P03.007.002) (Modulo)

Autore CNR

• IGEA D'AGNANO (Persona)

Incoming links:

Autore CNR di

• IGEA D'AGNANO (Persona)

Prodotto

• ex ME.P03.007.001 / Meccanismi regolativi del Differenziamento e Oncogenesi (ME.P03.007.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Molecular bioSystems (Rivista)