YAC-ASSISTED CLONING OF TRANSCRIBED SEQUENCES FROM THE HUMAN CHROMOSOME-3P21 REGION (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• YAC-ASSISTED CLONING OF TRANSCRIBED SEQUENCES FROM THE HUMAN CHROMOSOME-3P21 REGION (Articolo in rivista) (literal)

Anno

• 1993-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1093/hmg/2.6.791 (literal)

Alternative label

• Pengue G, Calabrò V, Cannada-Bartoli P, De Luca P, Esposito T, Taillon-Miller P, LaForgia S, Druck T, Huebner K, D'Urso M, et al. (1993)
  YAC-ASSISTED CLONING OF TRANSCRIBED SEQUENCES FROM THE HUMAN CHROMOSOME-3P21 REGION
  in Human molecular genetics (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Pengue G, Calabrò V, Cannada-Bartoli P, De Luca P, Esposito T, Taillon-Miller P, LaForgia S, Druck T, Huebner K, D'Urso M, et al. (literal)

Pagina inizio

• 791 (literal)

Pagina fine

• 796 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 2 (literal)

Rivista

• Human molecular genetics (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• [ 1 ] UNIV NAPLES,DIPARTIMENTO BIOL GEN & MOLEC,VIA MEZZOCANNONE 8,I-80134 NAPLES,ITALY [ 2 ] CNR,INT INST GENET & BIOPHYS,I-80125 NAPLES,ITALY Organization-Enhanced Name(s) Consiglio Nazionale delle Ricerche (CNR) [ 3 ] THOMAS JEFFERSON UNIV,JEFFERSON CANC INST,PHILADELPHIA,PA 19107 Organization-Enhanced Name(s) Thomas Jefferson University [ 4 ] WASHINGTON UNIV,SCH MED,CTR HUMAN GENOME,ST LOUIS,MO 63110 (literal)

Titolo

• YAC-ASSISTED CLONING OF TRANSCRIBED SEQUENCES FROM THE HUMAN CHROMOSOME-3P21 REGION (literal)

Abstract

• The region surrounding the ZNF35 zinc finger protein gene on 3p21 is of particular interest, as this region of chromosome 3 is frequently involved in rearrangements and/or deletions associated with various human tumors including lung and renal carcinoma. We have analyzed yeast artificial chromosomes (YACs), identified by PCR screening, using oligonucleotides derived from the ZNF35 gene. PFGE and Southern blot/hybridization analysis revealed that the clones cover 750-kb including the ZNF35 gene. The use of specific somatic cell hybrids have allowed us to locate the YAC contig telomeric to the D3F15S2 locus, in a region which is frequently deleted in lung carcinomas. In addition, we have developed a novel cDNA hybridization protocol allowing the isolation of transcribed sequences present in the overlapping YAC clones. Using the cDNA hybridization selection, we have isolated and characterized one transcribed sequence (D3S1362E) from the 3p21 YAC contig and the corresponding cDNA has been isolated. DNA sequencing analysis indicated that the D3S1363E cDNA codes for a putative transcription factor. Northern blot analysis indicated that the D3S1362E sequence hybridized to multiple transcripts in skeletal muscle, and weakly hybridizing transcripts of similar sizes were detected in other tissues. (literal)

Prodotto di

• Institute of genetics and biophysics \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Plasticità genomica: dal genoma ai sistemi biologici (SV.P13.003.001) (Modulo)

Autore CNR

• TERESA ESPOSITO (Persona)

Incoming links:

Prodotto

• Institute of genetics and biophysics \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Plasticità genomica: dal genoma ai sistemi biologici (SV.P13.003.001) (Modulo)

Autore CNR di

• TERESA ESPOSITO (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Human molecular genetics (Rivista)