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Properties of mixed DOTAP-DPPC bilayer membranes as reported by differential scanning calorimetry and dynamic light scattering measurements (Articolo in rivista)

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Properties of mixed DOTAP-DPPC bilayer membranes as reported by differential scanning calorimetry and dynamic light scattering measurements (Articolo in rivista) (literal)

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Cinelli, S; Onori, G; Zuzzi, S; Bordi, F; Cametti, C; Sennato, S; Bordi, F; Cametti, C; Sennato, S; Diociaiuti, M (2007)
Properties of mixed DOTAP-DPPC bilayer membranes as reported by differential scanning calorimetry and dynamic light scattering measurements
in The journal of physical chemistry. B
(literal)

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Cinelli, S; Onori, G; Zuzzi, S; Bordi, F; Cametti, C; Sennato, S; Bordi, F; Cametti, C; Sennato, S; Diociaiuti, M (literal)

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Univ Roma La Sapienza, Dipartimento Fis, I-00185 Rome, Italy; INFM CRS SOFT, Unita Roma 1, Rome, Italy; Ist Super Sanita, I-00185 Rome, Italy; Univ Perugia, Dipartimento Fis, I-06123 Perugia, Italy; CEMIN, I-06123 Perugia, Italy (literal)

Titolo

Properties of mixed DOTAP-DPPC bilayer membranes as reported by differential scanning calorimetry and dynamic light scattering measurements (literal)

Abstract

We have investigated the effect of a cationic lipid [DOTAP] on both the thermotropic phase behavior and the structural organization of aqueous dispersions of dipalmitoyl-phosphatidylcholine [DPPC] by means of high-sensitivity differential scanning calorimetry and dynamic light scattering measurements. We find that the incorporation of increasing quantities of DOTAP progressively reduces the temperature and the enthalpy of the gel-to-liquid crystalline transition. We are further showing that, in mixed DOTAP-DPPC systems, the reduction of the phase transition temperature is accompanied by a reduction of the average size of the structures present in the aqueous mixtures, whatever the DOTAP concentration is. These results, which extend a previous investigation by Campbell et al. (Campbell, R. B.; Balasubramanian, S. V.; Straubinger, R. M.; Biochim. Biosphys. Acta 2001, 27, 1512.) limited to a DOTAP concentration below 20 mol %, confirm that the insertion of cationic head groups in zwitterionic phosphatidylcholine bilayers facilitates the formation of stable, relatively small, unilamellar vesicles. This self-assembling restructuring from an aqueous multilamellar structure toward a liposomal phase is favored by decreasing the phospholipid phase transition temperature and by increasing the temperature of the system. This reduction of the average size and the appearance of a stable liposomal phase is also promoted by a heating and cooling thermal treatment. (literal)

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