http://www.cnr.it/ontology/cnr/individuo/prodotto/ID218265
Isolation of a trans-acting factor involved in localization of Paracentrotus lividus maternal messenger RNAs. (Articolo in rivista)
- Type
- Label
- Isolation of a trans-acting factor involved in localization of Paracentrotus lividus maternal messenger RNAs. (Articolo in rivista) (literal)
- Anno
- 1999-01-01T00:00:00+01:00 (literal)
- Alternative label
Costa C, Romancino DP, Ingrassia A, Vizzini A, Di Carlo M. (1999)
Isolation of a trans-acting factor involved in localization of Paracentrotus lividus maternal messenger RNAs.
in RNA (Camb., Print); Cambridge University Press, Cambridge (Regno Unito)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Costa C, Romancino DP, Ingrassia A, Vizzini A, Di Carlo M. (literal)
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- Note
- ISI Web of Science (WOS), Scopus (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- IBIM CNR
Di. Biol Animale UniPA (literal)
- Titolo
- Isolation of a trans-acting factor involved in localization of Paracentrotus lividus maternal messenger RNAs. (literal)
- Abstract
- Localization of Paracentrotus lividus bep maternal mRNAs at the animal pole occurs by association with the cytoskeleton
and involves a 54-kDa protein, called LP54, that is able to bind to the 39 untranslated regions (UTRs) of bep
mRNAs. We describe here the isolation and purification of this protein. Antibodies raised against purified LP54
allowed us to establish its localization in P. lividus eggs and embryos. This localization coincides with the mRNAs with
which it is associated, that is, the animal pole in the egg, and, after fertilization, the regions derived from this part of
the egg, and finally the oral ectoderm of the pluteus. Association with the cytoskeleton was shown by the copurification
of LP54 in a microtubule preparation. Involvement in bep mRNA localization was demonstrated by microinjection
of anti-LP54 antibodies in P. lividus eggs, which caused alteration of spatial distribution of bep3 mRNA. (literal)
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