http://www.cnr.it/ontology/cnr/individuo/prodotto/ID214982
A monoclonal antibody, R1, and a polyclonal serum, S10, recognize the same molecules: A novel use of DNA transfectants (Articolo in rivista)
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- Label
- A monoclonal antibody, R1, and a polyclonal serum, S10, recognize the same molecules: A novel use of DNA transfectants (Articolo in rivista) (literal)
- Anno
- 1988-01-01T00:00:00+01:00 (literal)
- Alternative label
Banting, G., Mondello, C., Hope, R., Goodfellow, P. (1988)
A monoclonal antibody, R1, and a polyclonal serum, S10, recognize the same molecules: A novel use of DNA transfectants
in Journal of immunogenetics
(literal)
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- Banting, G., Mondello, C., Hope, R., Goodfellow, P. (literal)
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- Banting, G., Mondello, C., Hope, R., Goodfellow, P: Imperial Cancer Research Fund (literal)
- Titolo
- A monoclonal antibody, R1, and a polyclonal serum, S10, recognize the same molecules: A novel use of DNA transfectants (literal)
- Abstract
- The monoclonal antibody R1 defines the product of the MIC5 locus which has been localized to the long arm of the human X chromosome in the same region as the fragile site FRAXA. DNA transfectants, selected by R1 on the fluorescence-activated cell sorter (FACS), have been used to demonstrate that a previously described polyclonal antiserum, S10 (Buck & Bodmer, 1976), recognizes the same cell surface molecule as R1. Immunoprecipitation from metabolically and surface-labelled cells has shown that this molecule is a Mr 200 K phosphoprotein which is synthesized as a Mr 180 K precursor and subsequently modified to a Mr 195 K extracellular form. A Mr 150 K molecule is coprecipitated with the Mr 200 K phosphoprotein, but is only detected in lysates of surface-labelled cells, raising the possibility that the 200 K molecule is a cell surface receptor and the 150 K molecule a ligand. (literal)
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