Telomere length in fibroblasts and blood cells from healthy centenarians (Articolo in rivista)

Type
Label
  • Telomere length in fibroblasts and blood cells from healthy centenarians (Articolo in rivista) (literal)
Anno
  • 1999-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1006/excr.1999.4398 (literal)
Alternative label
  • Mondello C, Petropoulou C, Monti D, Gonos ES, Franceschi C, Nuzzo F (1999)
    Telomere length in fibroblasts and blood cells from healthy centenarians
    in Experimental cell research
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Mondello C, Petropoulou C, Monti D, Gonos ES, Franceschi C, Nuzzo F (literal)
Pagina inizio
  • 234 (literal)
Pagina fine
  • 242 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 248 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 1 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Mondello C, Nuzzo F: istituto di geentica Molecoalre Monti D: Dipartimento di Scienze Biomediche, Università di Modena Franceschi C: Dipartimento di Biologia Sperimentale, Università di Bologna; INRCA, Ancona Petropoulou C,Gonos ES: Natl Hellen Res Fdn, Inst Biol Res & Biotechnol, Lab Mol & Cellular Aging, Athens 11635, Greece (literal)
Titolo
  • Telomere length in fibroblasts and blood cells from healthy centenarians (literal)
Abstract
  • Several lines of evidence indicate that telomere shortening during in vitro aging of human somatic cells plays a causal role in cellular senescence. A critical telomere length seems to be associated with the replicative block characterizing senescent cells. In this paper we analyzed the mean length of the terminal restriction fragments (TRF) in fibroblast strains from 4 healthy centenarians, that is, in cells aged in vivo, and from 11 individuals of different ages. No correlation between mean TRF length and donor age was found. As expected, telomere shortening was detected during in vitro propagation of centenarian fibroblasts, suggesting that in fibroblasts aged in vivo telomeres can be far from reaching a critical length. Accordingly, chromosome analysis did not show the presence of telomeric associations in early passage centenarian fibroblasts. In blood cells from various individuals, the expected inverse correlation between mean TRF length and donor age was found. In particular, a substantial difference (about 2 kb) between telomere length in the two cell types was observed in the same centenarian. Expression analysis of three senescence-induced genes, i.e., fibronectin, apolipoprotein J, and p21, revealed for only the fibronectin expression levels a clear positive correlation with donor age. Our results suggest that (1) telomere shortening could play a different role in the aging of different cell types and (2) the characteristics of fibroblasts aged in vitro might not be representative of what occurs in vivo. (literal)
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