UbcH10 expression on thyroid fine-needle aspirates. (Articolo in rivista)

Type
Label
  • UbcH10 expression on thyroid fine-needle aspirates. (Articolo in rivista) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
Alternative label
  • Guerriero E, Ferraro A, Desiderio D, Pallante P, Berlingieri MT, Iaccarino A, Palmieri E, Palombini L, Fusco A, Troncone G. (2010)
    UbcH10 expression on thyroid fine-needle aspirates.
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Guerriero E, Ferraro A, Desiderio D, Pallante P, Berlingieri MT, Iaccarino A, Palmieri E, Palombini L, Fusco A, Troncone G. (literal)
Pagina inizio
  • 157 (literal)
Pagina fine
  • 165 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 118 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Titolo
  • UbcH10 expression on thyroid fine-needle aspirates. (literal)
Abstract
  • BACKGROUND: Thyroid fine-needle aspiration (FNA) samples belonging to the follicular neoplasm/suspicious for malignancy classes are controversial. The authors identified UbcH10 as a marker useful in the diagnosis of several neoplasms, including thyroid cancer. Here, analysis of UbcH10 expression by quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemistry was applied to FNAs. METHODS: A series of 84 follicular neoplasm/suspicious for malignancy FNAs with histological follow-up (30 malignant) was prospectively collected. UbcH10 immunostaining was performed on cell blocks and compared with that of the proliferation marker Ki-67. At the mRNA level, UbcH10 was compared with CCND2 and PCSK2 expression, these latter being the best performing components of the previously reported 3-gene assay; to determine the diagnostic accuracy, the area under the curve (AUC) of the receiver operating characteristic (ROC) curve for each gene individually and in combination was evaluated. RESULTS: UbcH10 and Ki-67 shared a similar pattern; although UbcH10 expression was higher in malignant than in benign lesions (P < .001), staining was sporadic, and the cutoff value derived by the ROC analysis was too low (1.25%) for routine application. Conversely, UbcH10 expression assessment by quantitative RT-PCR was effective. UbcH10 mRNA levels associated with malignant histology were significantly higher than those associated with benign histology (P = .02). The AUC was 0.74 for UbcH10, 0.81 for CCDN2, 0.62 for PCSK2, and 0.84 for UbcH10 and CCND2 combination. CONCLUSIONS: UbcH10 quantitative RT-PCR analysis, rather than immunohistochemistry, is useful to increase the detection of malignancy in thyroid FNAs. UbcH10 may be added as a panel component in quantitative RT-PCR-based assays. (literal)
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