Direct interactions among the tyrosine kinase receptor Ret, the GDNF and the GFR±1 molecules. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Direct interactions among the tyrosine kinase receptor Ret, the GDNF and the GFR±1 molecules. (Articolo in rivista) (literal)

Anno

• 2005-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.cellsig.2004.10.012 (literal)

Alternative label

• Amoresano V; Incoronato M; Monti G; Pucci P; de Franciscis V; Cerchia L (2005)
  Direct interactions among the tyrosine kinase receptor Ret, the GDNF and the GFR±1 molecules.
  in Cellular signalling
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Amoresano V; Incoronato M; Monti G; Pucci P; de Franciscis V; Cerchia L (literal)

Pagina inizio

• 717 (literal)

Pagina fine

• 727 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 17 (literal)

Rivista

• Cellular signalling (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto per l'Endocrinologia e l'Oncologia Sperimentale del CNR G. Salvatore (literal)

Titolo

• Direct interactions among the tyrosine kinase receptor Ret, the GDNF and the GFR±1 molecules. (literal)

Abstract

• The glial-cell-line-derived neurotrophic factor (GDNF) ligand activates the Ret receptor through the assembly of a multiprotein complex, including the GDNF family receptor a1 (GFRa1) molecule. Given the neuroprotective role of GDNF, there is an obvious need to precisely identify the structural regions engaged in direct interactions between the three molecules. Here, we combined a functional approach for Ret activity (in PC12 cells) to cross-linking experiments followed by MS-MALDI to study the interactions among the purified extracellular region of the human Ret, GDNF and GFRa1 molecules. This procedure allowed us to identify distinct regions of Ret that are physically engaged in the interaction with GDNF and GFRa1. The lack of these regions in a recombinant Ret form results in the failure of both structural and functional binding of Ret to GFRa1/GDNF complex. Furthermore, a model for the assembly of a transducing-competent Ret complex is suggested. (literal)

Prodotto di

• Individuazione di bersagli molecolari per lo sviluppo di terapie per i tumori (SV.P15.012.001) (Modulo)
• Istituto per l'endocrinologia e l'oncologia \"Gaetano Salvatore\" (IEOS) (Istituto)

Autore CNR

• LAURA CERCHIA (Persona)
• VITTORIO DE FRANCISCIS (Persona)

Incoming links:

Autore CNR di

• LAURA CERCHIA (Persona)
• VITTORIO DE FRANCISCIS (Persona)

Prodotto

• Istituto per l'endocrinologia e l'oncologia \"Gaetano Salvatore\" (IEOS) (Istituto)
• Individuazione di bersagli molecolari per lo sviluppo di terapie per i tumori (SV.P15.012.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Cellular signalling (Rivista)