H4(D10S170), a gene frequently rearranged with RET in papillary thyroid carcinomas: functional characterization. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• H4(D10S170), a gene frequently rearranged with RET in papillary thyroid carcinomas: functional characterization. (Articolo in rivista) (literal)

Anno

• 2004-01-01T00:00:00+01:00 (literal)

Alternative label

• Celetti A; Cerrato A; Merolla F; Vitagliano D; Vecchio G; Grieco M. (2004)
  H4(D10S170), a gene frequently rearranged with RET in papillary thyroid carcinomas: functional characterization.
  in Oncogene (Basingstoke)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Celetti A; Cerrato A; Merolla F; Vitagliano D; Vecchio G; Grieco M. (literal)

Pagina inizio

• 109 (literal)

Pagina fine

• 121 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 23 (literal)

Rivista

• Oncogene (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto di Endocrinologia ed Oncologia Sperimentale del CNR c/o Dipartimento di Biologia e Patologia Cellulare e Molecolare, University 'Federico II', Naples, Italy; and Dipartimento di Medicina Sperimentale e Clinica, Universita` 'Magna Graecia', via T. Campanella 115, Policlinico Materdomini, Catanzaro, CZ 88100, Italy (literal)

Titolo

• H4(D10S170), a gene frequently rearranged with RET in papillary thyroid carcinomas: functional characterization. (literal)

Abstract

• Human thyroid papillary carcinomas are characterized by rearrangements of the RET protooncogene with a number of heterologous genes, which generate the RET/papillary thyroidcarcinoma(PTC)oncogenes.Oneofthemost frequent variants of these recombination events is the fusion of the intracellular kinase-encoding domain of RET to the first 101 amino acids of a gene named H4(D10S170).WehavecharacterizedtheH4(D10S170) gene product, showing that it is a ubiquitously expressed 55KDa nuclear and cytosolic protein that is phosphory- latedfollowingserumstimulation.Thisphosphorylation was found to depend on mitogen-activated protein kinase(MAPK)Erk1/2activityandtobeassociated totherelocationofH4(D10S170)fromthenucleusto the cytosol. Overexpression of the H4(D10S170) gene was able to induce apoptosis of thyroid follicular epithelial cells; conversely a carboxy-terminal truncated H4(D10S170) mutant H4(1-101), corresponding to the portion included in the RET/PTC1 oncoprotein, behaved as dominant negative on the proapoptotic function and nuclear localization of H4(D10S170). Furthermore, con- ditional expression of the H4(D10S170)-dominant nega- tive truncated mutant protected cells from stress-induced apoptosis. The substitution of serine 244 with alanine abrogated the apoptotic function of H4(D10S170). These data suggest that loss of the H4(D10S170) gene function might have a role in thyroid carcinogenesis by impairing apoptosis. (literal)

Prodotto di

• Istituto per l'endocrinologia e l'oncologia \"Gaetano Salvatore\" (IEOS) (Istituto)
• Identificazione e caratterizzazione funzionale dei geni e delle proteine coinvolti nella tumorigenesi tiroidea (SV.P15.001.004) (Modulo)

Autore CNR

• ANIELLO CERRATO (Persona)
• ANGELA CELETTI (Persona)

Incoming links:

Autore CNR di

• ANGELA CELETTI (Persona)
• ANIELLO CERRATO (Persona)

Prodotto

• Istituto per l'endocrinologia e l'oncologia \"Gaetano Salvatore\" (IEOS) (Istituto)
• Identificazione e caratterizzazione funzionale dei geni e delle proteine coinvolti nella tumorigenesi tiroidea (SV.P15.001.004) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Oncogene (Rivista)