Functional expression of the CXCR4 chemokine receptor is induced by RET/PTC oncogenes and is a common event in human papillary thyroid carcinomas. (Articolo in rivista)

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  • Functional expression of the CXCR4 chemokine receptor is induced by RET/PTC oncogenes and is a common event in human papillary thyroid carcinomas. (Articolo in rivista) (literal)
Anno
  • 2004-01-01T00:00:00+01:00 (literal)
Alternative label
  • Castellone MD; Guarino V; De Falco V; Carlomagno F; Basolo F; Faviana P; Kruhoffer M; Orntoft T; Russell JP; Rothstein JL; Fusco A; Santoro M; Melillo RM. (2004)
    Functional expression of the CXCR4 chemokine receptor is induced by RET/PTC oncogenes and is a common event in human papillary thyroid carcinomas.
    in Oncogene (Basingstoke)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Castellone MD; Guarino V; De Falco V; Carlomagno F; Basolo F; Faviana P; Kruhoffer M; Orntoft T; Russell JP; Rothstein JL; Fusco A; Santoro M; Melillo RM. (literal)
Pagina inizio
  • 5958 (literal)
Pagina fine
  • 5967 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 23 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Castellone MD; Guarino V; De Falco V; Carlomagno F, Istituto di Endocrinologia ed Oncologia Sperimentale del CNR 'G Salvatore', c/o Dipartimento di Biologia e Patologia Cellulare e Molecolare, 80131 Naples, Italy; Basolo F; Faviana P, Dipartimento di oncologia, Pisa, Italy; Kruhoffer M; Orntoft T, Molecular Diagnostic Laboratory, Department of Clinical Biochemistry, Aarhus University Hospital, Skejby, Aarhus, Denmark; Russell JP; Rothstein JL, Departments of Microbiology/Immunology and Otolaryngology-Head and neck surgery, Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, PA 19107, USA; Fusco A; Santoro M; Melillo RM,Istituto di Endocrinologia ed Oncologia Sperimentale del CNR 'G Salvatore', c/o Dipartimento di Biologia e Patologia Cellulare e Molecolare, 80131 Naples, Italy; (literal)
Titolo
  • Functional expression of the CXCR4 chemokine receptor is induced by RET/PTC oncogenes and is a common event in human papillary thyroid carcinomas. (literal)
Abstract
  • To identify genes involved in the transformation of thyroid follicular cells, we explored, using DNA oligonucleotide microarrays, the transcriptional response of PC Cl3 rat thyroid epithelial cells to the ectopic expression of the RET/PTC oncogenes. We found that RET/PTC was able to induce the expression of CXCR4, the receptor for the chemokine CXCL12/SDF-1a/b. We observed that CXCR4 expression correlated with the transforming ability of the oncoprotein and depended on the integrity of the RET/PTC-RAS/ERK signaling pathway. We found that CXCR4 was expressed in RET/PTC-positive human thyroid cancer cell lines, but not in normal thyroid cells. Furthermore, we found CXCR4 expression in human thyroid carcinomas, but not in normal thyroid samples by immunohistochemistry. Since CXCR4 has been recently implicated in tumor proliferation, motility and invasiveness, we asked whether treatment with SDF-1a was able to induce a biological response in thyroid cells. We observed that SDF-1a induced S-phase entry and survival of thyroid cells. Invasion through a reconstituted extracellular matrix was also supported by SDF-1a and inhibited by a blocking antibody to CXCR4. Taken together, these results suggest that human thyroid cancers bearing RET/PTC rearrangements may use the CXCR4/ SDF-1a receptor-ligand pathway to proliferate, survive and migrate. (literal)
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