Two-photon microscopy of aorta fibers shows proteolysis induced by LDL hydroperoxides (Articolo in rivista)

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  • Two-photon microscopy of aorta fibers shows proteolysis induced by LDL hydroperoxides (Articolo in rivista) (literal)
Anno
  • 2000-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/S0891-5849(00)00275-6 (literal)
Alternative label
  • Parasassi T, Yu W, Durbin D, Kuriashkina L, Gratton E, Maeda N, Ursini F (2000)
    Two-photon microscopy of aorta fibers shows proteolysis induced by LDL hydroperoxides
    in Free radical biology & medicine
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Parasassi T, Yu W, Durbin D, Kuriashkina L, Gratton E, Maeda N, Ursini F (literal)
Pagina inizio
  • 1589 (literal)
Pagina fine
  • 1587 (literal)
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  • 28 (literal)
Rivista
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  • 11 (literal)
Note
  • ISI Web of Science (WOS) (literal)
  • PubMed (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Istituto di Farmacologia Traslazionale, CNR Laboratory for Fluorescence Dynamics, Department of Physics, University of Illinois at Urbana-Champaign, IL USA Department of Biochemistry, University of Illinois College of Medicine at Urbana-Champaign, IL, USA Department of Molecular and Integrative Physiology, University of Illinois at Urbana-Champaign, IL, USA Department of Pathology, School of Medicine, University of North Carolina, Chapel Hill, NC, USA Dipartimento di Chimica Biologica, Universita' di Padova (literal)
Titolo
  • Two-photon microscopy of aorta fibers shows proteolysis induced by LDL hydroperoxides (literal)
Abstract
  • Oxidatively modified LDL mimics several aspects of atherogenesis. In this disease, degradation of the matrix proteins' network also occurs. By a new morphological ex vivo approach, not requiring sample processing, we explored the relationship between the degradation of matrix protein and oxidatively modified LDL. Two-photon excitation fluorescence microscopy images of fresh cross-section rings of rat aorta, acquired while the sample was maintained in a glucose- and oxygen-supplemented buffer, showed straight, parallel, thick, long extracellular matrix proteins. Traditional microscopic examination, requiring sample fixation and staining, shows smaller and curved fibers. Instead, we observed curved and broken fibers after a 30-min incubation of aorta with either LDL containing lipid hydroperoxides, or tert-butyl-hydroperoxide. The adhesion of LDL to the endothelium and its internalization was directly visualized by using a lipid fluorophore. The damage to aorta matrix proteins induced by LDL and tert-butylhydroperoxide was fully prevented by antioxidants, such as ascorbate or Trolox C, or inhibitors of proteases. The image spectroscopy of the fibers' autofluorescence (polarization and lifetime) revealed an increased mobility of the fluorescent cross-link in fibers. Damaged matrix proteins were also imaged in aorta samples from apolipoprotein E knock-out mice. Our ex vivo images directly visualized the activation of a fast redox-sensitive proteolytic process in the arterial wall triggered by lipid hydroperoxides in LDL. (literal)
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