http://www.cnr.it/ontology/cnr/individuo/prodotto/ID211097
Lazaroid U-74389F attenuates phorbol ester-induced lung injury in rabbits (Articolo in rivista)
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- Label
- Lazaroid U-74389F attenuates phorbol ester-induced lung injury in rabbits (Articolo in rivista) (literal)
- Anno
- 1996-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1183/09031936.96.09040758 (literal)
- Alternative label
M. Miniati*, F. Cocci**, S. Monti*, E. Filippi*, R. Sarnelli+, M. Ferdeghini++, V. Gattai*, M. Pistolesi# (1996)
Lazaroid U-74389F attenuates phorbol ester-induced lung injury in rabbits
in The European respiratory journal
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- M. Miniati*, F. Cocci**, S. Monti*, E. Filippi*, R. Sarnelli+, M. Ferdeghini++, V. Gattai*, M. Pistolesi# (literal)
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- http://erj.ersjournals.com/content/9/4/758.full.pdf+html (literal)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
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- ISI Web of Science (WOS) (literal)
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- *Istituto di Fisiologia Clinica del CNR, **Istituto di Patologia Medica I, #Istituto di Clinica Medica II, ++Centro Regionale di Medicina Nucleare, Università di Pisa, and +Scuola Superiore di Studi Universitarie Perfezionamento \"S. Anna\", Pisa, Italy. (literal)
- Titolo
- Lazaroid U-74389F attenuates phorbol ester-induced lung injury in rabbits (literal)
- Abstract
- We examined the effect of the antioxidant lazaroid U-74389F on acute
lung injury induced in rabbits by phorbol myristate acetate (PMA).
Thirty minutes after receiving either U-74389F (15 mg·kg-1 i.v.) or U-74389F vehicle,
rabbits (n=60) were given PMA (60 ?g·kg-1 i.v.). PMA vehicle injected rabbits
(n=20) served as controls. Over a 5 h period after PMA or PMA vehicle injection,
we measured arterial pH, arterial oxygen tension (Pa,O2), arterial carbon dioxide
tension (Pa,CO2), and the plasma concentration of the neutrophil chemoattractant
interleukin-8 (IL-8). At postmortem, lungs were inspected for macroscopic injury
and examined histologically. Malondialdehyde levels were assayed in lung tissue as
an index of lipid peroxidation. In bronchoalveolar lavage (BAL), total and differential
cell counts, protein and IL-8 concentrations were measured.
Compared to normal controls, rabbits challenged with PMA alone developed arterial
acidosis, hypercapnia and hypoxaemia, accompanied by significant rise in
plasma IL-8 concentration. U-74389F pretreated animals did not develop significant
arterial blood gas abnormalities and had significantly lower IL-8 concentration
in plasma. U-74389F did not prevent PMA-induced lipid peroxidation. However,
macroscopic signs of lung injury and the degree of alveolar haemorrhage and protein
extravasation were significantly less severe in pretreated rabbits than in those
given PMA alone. In addition, U-74389F significantly reduced IL-8 concentration
and neutrophil number in BAL. By histological assessment, 80% of lung neutrophils
were localized in alveolar spaces of animals receiving PMA alone. Conversely, in
U-74389F pretreated animals, 75% of neutrophils were distributed within extraalveolar
blood vessels and alveolar septa.
We conclude that lazaroid U-74389F attenuates lung injury in rabbits given PMA
by preventing neutrophil migration into pulmonary alveoli. This effect may, in
part, be related to downregulation of IL-8 production. (literal)
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