Mitochondria act as a reservoir for the basic amine HIPDM in the lung (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Mitochondria act as a reservoir for the basic amine HIPDM in the lung (Articolo in rivista) (literal)

Anno

• 1996-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1183/09031936.96.09112306 (literal)

Alternative label

• M. Miniati*, A. Paci**, F. Cocci**, G. Ciarimboli*, S. Monti*, M. Pistolesi+ (1996)
  Mitochondria act as a reservoir for the basic amine HIPDM in the lung
  in The European respiratory journal
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• M. Miniati*, A. Paci**, F. Cocci**, G. Ciarimboli*, S. Monti*, M. Pistolesi+ (literal)

Pagina inizio

• 2306 (literal)

Pagina fine

• 2312 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://erj.ersjournals.com/content/9/11/2306.full.pdf+html (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 9 (literal)

Rivista

• ?The ?European respiratory journal (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 7 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 11 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• *Istituto di Fisiologia Clinica del CNR, **Istituto di Patologia Medica I, +Istituto di Clinica Medica II, Università di Pisa, Pisa, Italy. (literal)

Titolo

• Mitochondria act as a reservoir for the basic amine HIPDM in the lung (literal)

Abstract

• The lungs are a site for the uptake, accumulation, and storage of exogenous basic amines. The compound N-N-N'-trimethyl-N'-(2-hydroxy-3-methyl-5-iodobenzyl)-1,3 propanediamine (HIPDM) is a basic amine that can be labelled with radioactive iodine and detected by external counting, Intravenously injected I-123-HIPDM is extracted by the human lung, where it is retained in a slowly effuxable pool. In the present study, we measured HIPDM lung kinetics and subcellular distribution in rabbits given i.v. I-125-HIPDM. Rabbits were killed from 2 min to 5 h after injection, and the radioactivity retained in their lungs was measured, Subcellular lung fractions (nuclear, mitochondrial, lysosomal, microsomal, and postmicrosomal supernatant) were assayed for HIPDM radioactivity, protein contene, and distribution of specific marker enzymes. HIPDM lung clearance in rabbits was nearly identical to that of humans, Virtually all the HIPDM radioactivity in lungs (98+1%) was associated with subcellular membranous structures. The highest HIPDM specific radioactivity was found in the mitochondrial Fraction, and the subcellular distribution profile closely resembled that of the mitochondrial marker enzyme succinate cytochrome c reductase, No redistribution of HIPDM among subcellular compartments was observed over a 5 h period after injection. The data indicate that mitochondria act as reservoir for HIPDM in the lungs and contribute to the pulmonary persistence of this compound, HIPDM can be used to investigate the pulmonary uptake of basic amines in health and in lung disease. (literal)

Prodotto di

• Istituto di fisiologia clinica (IFC) (Istituto)

Autore CNR

• MASSIMO MINIATI (Unità di personale interno)
• SIMONETTA MONTI (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Mitochondria act as a reservoir for the basic amine HIPDM in the lung" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• SIMONETTA MONTI (Unità di personale interno)
• MASSIMO MINIATI (Unità di personale interno)

Prodotto

• Istituto di fisiologia clinica (IFC) (Istituto)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• ?The ?European respiratory journal (Rivista)

Insieme di parole chiave di

• Keywords of "Mitochondria act as a reservoir for the basic amine HIPDM in the lung" (Insieme di parole chiave)