http://www.cnr.it/ontology/cnr/individuo/prodotto/ID21093
Specific microRNAs are downregulated in human thyroid anaplastic carcinomas. (Articolo in rivista)
- Type
- Label
- Specific microRNAs are downregulated in human thyroid anaplastic carcinomas. (Articolo in rivista) (literal)
- Anno
- 2008-01-01T00:00:00+01:00 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Visone R; Pallante P; Vecchione A; Cirombella R; Ferracin M; Ferraro A; Volinia S; Coluzzi S; Leone V; Borbone E; Liu CG; Petrocca F; Troncone G; Calin GA; Scarpa A; Colato C; Tallini G; Santoro M; Croce CM; Fusco A. (literal)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
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- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Dipartimento di Biologia e Patologia Cellulare e Molecolare c/o Istituto di Endocrinologia ed Oncologia Sperimentale del CNR,
Facolta` di Medicina e Chirurgia, Universita` degli Studi di Napoli 'Federico II', Naples, Italy;
NOGEC (Naples Oncogenomic
Center)-CEINGE, Biotecnologie Avanzate-Napoli, & SEMM - European School of Molecular Medicine - Naples Site, Naples, Italy;
Division of Human Cancer Genetics, Comprehensive Cancer Center, Ohio State University, Columbus, OH, USA; Dipartimento di
Istopatologia, Ospedale Sant'Andrea, Universita` di Roma 'La Sapienza', Rome, Italy; 5Dipartimento di Medicina Sperimentale e
Diagnostica, Centro Interdipartimentale per la Ricerca sul Cancro, Universita` di Ferrara, Ferrara, Italy; 6Dipartimento di Anatomia
Patologica e Citopatologia, Facolta` di Medicina e Chirurgia, Universita` degli Studi di Napoli 'Federico II', Naples, Italy;
7Dipartimento di Patologia, Universita` di Verona, Verona, Italy and 8Dipartimento di Patologia, Universita` di Bologna - Ospedale
Bellaria, Bologna, Italy
Thyroid carcinomas comprise a broad spectrum oftumors
with different clinical behaviors. On the one side, there are
occult papillary carcinomas (PTC), slow growing and
clinically silent, and on the other side, rapidly growing
anaplastic carcinomas (ATC), which are among the most
lethal human neoplasms. We have analysed the micro-
RNA (miR) profile ofATC in comparison to the normal
thyroid using a microarray (miRNACHIP microarray).
By this approach, we found an aberrant miR expression
profile that clearly differentiates ATC from normal
thyroid tissues and from PTC analysed in previous
studies. In particular, a significant decrease in miR-30d,
miR-125b, miR-26a and miR-30a-5p was detected in ATC
in comparison to normal thyroid tissue. These results were
further confirmed by northern blots, quantitative reverse
transcription-PCR analyses and in situ hybridization. The
overexpression ofthese four miRs in two human ATCderived
cell lines suggests a critical role ofmiR- 125b and
miR-26a downregulation in thyroid carcinogenesis, since a
cell growth inhibition was achieved. Conversely, no effect
on cell growth was observed after the overexpression of
miR-30d and miR-30a-5p in the same cells. In conclusion,
these data indicate a miR signature associated with ATC
and suggest the miR deregulation as an important event in
thyroid cell transformation.
Oncogene advance online publication, 11 June 2007;
doi:10.1038/sj.onc.1210564
Keywords: microRNA; (literal)
- Titolo
- Specific microRNAs are downregulated in human thyroid anaplastic carcinomas. (literal)
- Abstract
- Thyroid carcinomas comprise a broad spectrum oftumors
with different clinical behaviors. On the one side, there are
occult papillary carcinomas (PTC), slow growing and
clinically silent, and on the other side, rapidly growing
anaplastic carcinomas (ATC), which are among the most
lethal human neoplasms. We have analysed the micro-
RNA (miR) profile ofATC in comparison to the normal
thyroid using a microarray (miRNACHIP microarray).
By this approach, we found an aberrant miR expression
profile that clearly differentiates ATC from normal
thyroid tissues and from PTC analysed in previous
studies. In particular, a significant decrease in miR-30d,
miR-125b, miR-26a and miR-30a-5p was detected in ATC
in comparison to normal thyroid tissue. These results were
further confirmed by northern blots, quantitative reverse
transcription-PCR analyses and in situ hybridization. The
overexpression ofthese four miRs in two human ATCderived
cell lines suggests a critical role ofmiR- 125b and
miR-26a downregulation in thyroid carcinogenesis, since a
cell growth inhibition was achieved. Conversely, no effect
on cell growth was observed after the overexpression of
miR-30d and miR-30a-5p in the same cells. In conclusion,
these data indicate a miR signature associated with ATC
and suggest the miR deregulation as an important event in
thyroid cell transformation. (literal)
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