Replication mediated instability of the GAA triplet repeat mutation in Friedreich ataxia (Articolo in rivista)

Type
Label
  • Replication mediated instability of the GAA triplet repeat mutation in Friedreich ataxia (Articolo in rivista) (literal)
Anno
  • 2004-01-01T00:00:00+01:00 (literal)
Alternative label
  • Pollard L., Sharma R., Gomez M., Shah S., Delatycki M., Pianese L., Monticelli A., Keats B. and Bidichandani S. (2004)
    Replication mediated instability of the GAA triplet repeat mutation in Friedreich ataxia
    in Nucleic acids research
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Pollard L., Sharma R., Gomez M., Shah S., Delatycki M., Pianese L., Monticelli A., Keats B. and Bidichandani S. (literal)
Pagina inizio
  • 5962 (literal)
Pagina fine
  • 5971 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 39 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 12 (literal)
Note
  • ISI Web of Science (WOS) (literal)
  • Scopu (literal)
  • Google Scholar (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Department of Biochemistry and Molecular Biology and 2Department of Pediatrics, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA Bruce Leroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Department of Pediatrics, University of Melbourne Australia, 5BioGeM Consortium IEOS-CNR Naples, Italy DBPCM, Federico II University, Naples, Italy Department of Genetics, Louisiana State University Health Sciences Center, New Orleans, LA, USA (literal)
Titolo
  • Replication mediated instability of the GAA triplet repeat mutation in Friedreich ataxia (literal)
Abstract
  • Friedreich ataxia is caused by the expansion of a polymorphic and unstable GAA triplet repeat in the FRDA gene, but the mechanisms for its instability are poorly understood. Replication of (GAA*TTC)n sequences (9-105 triplets) in plasmids propagated in Escherichia coli displayed length- and orientation-dependent instability. There were small length variations upon replication in both orientations, but large contractions were frequently observed when GAA was the lagging strand template. DNA replication was also significantly slower in this orientation. To evaluate the physiological relevance of our findings, we analyzed peripheral leukocytes from human subjects carrying repeats of similar length (8-107 triplets). Analysis of 9400 somatic FRDA molecules using small-pool PCR revealed a similar mutational spectrum, including large contractions. The threshold length for the initiation of somatic instability in vivo was between 40 and 44 triplets, corresponding to the length of a eukaryotic Okazaki fragment. Consistent with the stabilization of premutation alleles during germline transmission, we also found that instability of somatic cells in vivo and repeats propagated in E.coli were abrogated by (GAGGAA)n hexanucleotide interruptions. Our data demonstrate that the GAA triplet repeat mutation in Friedreich ataxia is destabilized, frequently undergoing large contractions, during DNA replication. (literal)
Prodotto di
Autore CNR

Incoming links:


Autore CNR di
Prodotto
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
data.CNR.it