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Regeneration of Algerian Citrus germplasm by stigma/style somatic embryogenesis (Articolo in rivista)

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Regeneration of Algerian Citrus germplasm by stigma/style somatic embryogenesis (Articolo in rivista) (literal)

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Meziane M, Boudjeniba M, Frasheri D, D'Onghia AM, Carra A, Carimi F, Haddad N, Boukhalfa S, Braneci S (2012)
Regeneration of Algerian Citrus germplasm by stigma/style somatic embryogenesis
in African journal of biotechnology; Academic Journals, "Nairobi ; Lagos" (Kenya)
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Meziane M, Boudjeniba M, Frasheri D, D'Onghia AM, Carra A, Carimi F, Haddad N, Boukhalfa S, Braneci S (literal)

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Université Hassiba Benbouali de Chlef, Hay Salem, 19 route nationale, 02000 Chlef, Algérie; Laboratoire des Cultures in vitro. Ens Kouba B. P. 92, Alger, Algeria; Centre International de Hautes Etudes Agronomiques Méditerranéennes (CIHEAM)/Mediterranean Agronomic Institute. Via Ceglie 9, 70010 Valenzano (BARI), Italy; Institute of Plant Genetics - CNR, Research Division of Palermo. Corso Calatafimi 414, I- 90129 Palermo, Italy; Institut Technique de l'Arboriculture Fruitière et de la Vigne (ITAFV). Tassala-El Merdja, Birtouta, Algeria (literal)

Titolo

Regeneration of Algerian Citrus germplasm by stigma/style somatic embryogenesis (literal)

Abstract

Stigma/style somatic embryogenesis is one of the efficient methods in plant regeneration of most Citrus ssp., without inducing somaclonal variations. Furthermore, somatic embryogenesis from style/stigma proved to be effective in the elimination of the main citrus virus and virus-like diseases. This technique was applied on Algerian citrus collection. Different Citrus species [Citrus sinensis (L.) Osbeck, C. limon (L.) Burm, C. reticulata Blanco, C. paradisi Macfad, C. reshni Hort. ex Tan., C. jambhiri Lush and C. maxima (Burm.) Merrill] were chosen and tested for the presence of the main virus and virus-like agents. Most of the genotypes showed to be infected, mainly by viroid agents. Closed flowers were collected and in vitro cultured on a Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine. All explants produced callus about 4 to 9 days after culture initiation, whereas embryogenesis occurred after 38 to 150 days in most of the cultured genotypes. Formed embryos were cultured in a single tube before in vivo acclimatization. After sanitary assays, regenerated plants were shown to be free from the agents detected in the mother trees. (literal)

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