Structure and biological evaluation of amino-functionalized PVP nanogels for fast cellular internalization (Articolo in rivista)

Type
Label
  • Structure and biological evaluation of amino-functionalized PVP nanogels for fast cellular internalization (Articolo in rivista) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.reactfunctpolym.2013.04.011 (literal)
Alternative label
  • C. Dispenza, S. Rigogliuso, N. Grimaldi, M.A. Sabatino, D. Bulone, M.L. Bondì, G. Ghersi (2013)
    Structure and biological evaluation of amino-functionalized PVP nanogels for fast cellular internalization
    in Reactive & functional polymers (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • C. Dispenza, S. Rigogliuso, N. Grimaldi, M.A. Sabatino, D. Bulone, M.L. Bondì, G. Ghersi (literal)
Pagina inizio
  • 1103 (literal)
Pagina fine
  • 1113 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 73 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 11 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Dipartimento di Ingegneria Chimica, Gestionale, Informatica, Meccanica, Universita` degli Studi di Palermo, Viale delle Scienze, Ed. 6, 90128 Palermo, Italy Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche, Universita` degli Studi di Palermo, Viale delle Scienze, Ed. 16, 90128 Palermo, Italy CNR - Istituto di Biofisica (IBF) UOS Palermo, via Ugo La Malfa, 153, 90146 Palermo, Italy CNR - Istituto per lo Studio dei Materiali Nanostrutturati (ISMN) UOS Palermo, via Ugo La Malfa, 153, 90146 Palermo, Italy (literal)
Titolo
  • Structure and biological evaluation of amino-functionalized PVP nanogels for fast cellular internalization (literal)
Abstract
  • Aminopropyl methacrylamide chloride-graft-poly(N-vinyl pyrrolidone) nanogels (NGs) were designed to exploit the favorable properties of poly(N-vinyl pyrrolidone) (PVP), such as its high affinity to water and complexation ability of ions, molecules and macromolecules, with the availability of primary amino groups for bioconjugation reactions. A thorough structural characterization of the nanoscalar networks was performed via 1H-NMR and solid state 13C-NMR spectroscopies, while solid state NMR relaxation time measurements completed the NGs description in terms of polymer network density. Information on the hydrodynamic size and surface charge densities were sought via dynamic light scattering (DLS) and ?-potential measurements. These measurements, carried out both in water and in buffer and at the variance of pH, temperature and time, support the excellent colloidal stability of the amino-graft PVP nanogels and their redispersability from the dry physical form. The total absence of in-vitro toxicity and immunogenicity was ascertained first, and then cell localization studies in cell cultures offluorescent variants were performed. Furthermore, the possibility of decorating the nanogels with bovine serum albumin (BSA) to obtain a stable bio-hybrid colloidal nanoconstruct was established. All the gathered evidences qualify these materials as potential candidates to be used as nanocarriers for intra-cellular delivery of protein and other biomolecular drugs (literal)
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