Generation and characterization of novel monoclonal antibodies to the Ret receptor tyrosine kinase. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Generation and characterization of novel monoclonal antibodies to the Ret receptor tyrosine kinase. (Articolo in rivista) (literal)

Anno

• 2002-01-01T00:00:00+01:00 (literal)

Alternative label

• Salvatore G. 1-2, Nagata S. 1, Billaud M. 3, Santoro M. 2, Vecchio G. 2, Pastan I. 1 (2002)
  Generation and characterization of novel monoclonal antibodies to the Ret receptor tyrosine kinase.
  in Biochemical and biophysical research communications (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Salvatore G. 1-2, Nagata S. 1, Billaud M. 3, Santoro M. 2, Vecchio G. 2, Pastan I. 1 (literal)

Pagina inizio

• 813 (literal)

Pagina fine

• 817 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 294 (literal)

Rivista

• Biochemical and biophysical research communications (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Titolo

• Generation and characterization of novel monoclonal antibodies to the Ret receptor tyrosine kinase. (literal)

Abstract

• Ret is a tyrosine kinase receptor involved in several human diseases germ-line mutations are responsible for multiple endocrine neoplasia type 2 syndromes while somatic mutations of Ret are found in sporadic medullary thyroid carcinomas. In the present work, we describe the generation and characterization of a panel of novel monoclonal antibodies to Ret obtained by immunizing mice with a Ret-FC fusion protein. Fifty-five independent monoclonal antibodies recognize Ret-FC by enzyme linked immunosorbent assay but not a non-related FC fusion protein. Twenty antibodies further characterized recognize Ret expressing cells by flow cytometry. Finally, immunoprecipitation analysis showed that these antibodies recognize Ret mature glycosylated and immature forms. Thus, these monoclonal antibodies could be used as diagnostic tools to detect Ret expression, as well as therapeutic tools to downmodulate Ret or to deliver cytotoxic drugs to malignancies that overexpress Ret as neuroblastomas, medullary and papillary thyroid carcinomas, seminomas, and leukemia. (literal)

Prodotto di

• Institute Experimental Endocrinology and Oncology \"Gaetano Salvatore\" (IEOS) (Istituto)

Autore CNR

• MASSIMO SANTORO (Persona)
• GIULIANA SALVATORE (Persona)

Incoming links:

Autore CNR di

• GIULIANA SALVATORE (Persona)
• MASSIMO SANTORO (Persona)

Prodotto

• Institute Experimental Endocrinology and Oncology \"Gaetano Salvatore\" (IEOS) (Istituto)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Biochemical and biophysical research communications (Rivista)