Rapid Chagas diagnosis in clinical settings using a multiparametric assay. (Articolo in rivista)

Type
Label
  • Rapid Chagas diagnosis in clinical settings using a multiparametric assay. (Articolo in rivista) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Alternative label
  • Pierimarchi P*, Cerni L §, Alarcón de Noya B, Nicotera G*, Díaz-Bello Z, Angheben A, Scacciatelli D*, Zonfrillo M*, Recinelli G, Serafino A*. (2013)
    Rapid Chagas diagnosis in clinical settings using a multiparametric assay.
    in Diagnostic microbiology and infectious disease
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Pierimarchi P*, Cerni L §, Alarcón de Noya B, Nicotera G*, Díaz-Bello Z, Angheben A, Scacciatelli D*, Zonfrillo M*, Recinelli G, Serafino A*. (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • §) Byo Research S.r.l., Area Science Park, 16305, Basovizza, Trieste, Italy. *) Institute of Traslational Pharmacology- National Research Council of Italy (literal)
Titolo
  • Rapid Chagas diagnosis in clinical settings using a multiparametric assay. (literal)
Abstract
  • The development of an immunoassay for detecting circulating antibodies against Trypanosoma cruzi with a good performance appears to be crucial in clinical settings for the management of Chagas disease. Here we propose a new automated ELISA test performed on serum samples using 2 different T. cruzi antigens (a recombinant protein and a T. cruzi whole extract) placed in parallel in separate solid phases. This automated diagnostic tool allows the simultaneous analysis of a large number of sera, by determining the presence of antibodies against both antigens by a single run test, with high sensitivity and specificity. The simultaneous analysis of the reactivity against the 2 antigens in a biparametric modality reduces the percentage of false-negative sera and allows a more accurate diagnosis. Using this multiparametric approach, we propose an effective algorithm for the first step of Chagas diagnosis by performing a single test, with time and cost savings. (literal)
Prodotto di
Autore CNR

Incoming links:


Autore CNR di
Prodotto
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
data.CNR.it