Critical role of the HMGI(Y) proteins in adipocytic cell growth and differentiation. (Articolo in rivista)

Type
Label
  • Critical role of the HMGI(Y) proteins in adipocytic cell growth and differentiation. (Articolo in rivista) (literal)
Anno
  • 2001-01-01T00:00:00+01:00 (literal)
Alternative label
  • Melillo R.M. , Pierantoni G.M. , Scala S. , Battista S. , Fedele M. , Stella A. , De Biasio M.C. , Chiappetta G. , Fidanza V. , Condorelli G. , Santoro M. , Croce C.M. , Viglietto G. , Fusco A. (2001)
    Critical role of the HMGI(Y) proteins in adipocytic cell growth and differentiation.
    in Molecular and cellular biology (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Melillo R.M. , Pierantoni G.M. , Scala S. , Battista S. , Fedele M. , Stella A. , De Biasio M.C. , Chiappetta G. , Fidanza V. , Condorelli G. , Santoro M. , Croce C.M. , Viglietto G. , Fusco A. (literal)
Pagina inizio
  • 2485 (literal)
Pagina fine
  • 2495 (literal)
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  • Impact Factor = 9,836; Interdisciplinarietà del prodotto: The Authors belong to different scientific areas, sectors and Istitutions. The paper was in collaboration with International Institutions. (literal)
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  • 21 (literal)
Rivista
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  • The high-mobility group I (HMGI) nonhistone chromosomal proteins HMGI(Y) and HMGI-C have been implicated in defining chromatin structure and in regulating the transcription of several genes. These proteins have been implicated in adipocyte homeostasis: a severe deficiency of fat tissue is found in mice with targeted disruption of the HMGI-C locus, and lipomagenesis in humans is frequently associated with somatic mutations of HMGI genes. The aim of this study was to examine the role of HMGI(Y) proteins in adipocytic cell growth and differentiation. First, we found that differentiation of the preadipocytic 3T3-L1 cell line caused early induction of HMGI(Y) gene expression. Suppression of HMGI(Y) expression by antisense technology dramatically increased the growth rate and impaired adipocytic differentiation in these cells. The process of adipogenic differentiation involves the interplay of several transcription factors, among which is the CCAAT/enhancer-binding protein (C/EBP) family of proteins. We also tested the hypothesis that HMGI(Y) might participate in transcriptional control of adipocyte-specific promoters. We found that HMGI(Y) proteins bind C/EBPbeta in vivo and in vitro. Furthermore, we show that HMGI(Y) strongly potentiates the capacity of C/EBPbeta to transactivate the leptin promoter, an adipose-specific promoter. Taken together, these results indicate that the HMGI(Y) proteins play a critical role in adipocytic cell growth and differentiation. (literal)
Note
  • ISI Web of Science (WOS) (literal)
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  • Centro di Endocrinologia ed Oncologia Sperimentale del CNR, Dipartimento di Biologia e Patologia Cellulare e Molecolare, Università degli Studi di Napoli, INT Fondazione Senatore Pascale, Naples, Dipartimento di Medicina Sperimentale e Clinica, Università degli Studi di Catanzaro, Italy, Kimmel Cancer Center, Jefferson Medical College, Philadelphia, USA (literal)
Titolo
  • Critical role of the HMGI(Y) proteins in adipocytic cell growth and differentiation. (literal)
Abstract
  • The high-mobility group I (HMGI) nonhistone chromosomal proteins HMGI(Y) and HMGI-C have been implicated in defining chromatin structure and in regulating the transcription of several genes. These proteins have been implicated in adipocyte homeostasis: a severe deficiency of fat tissue is found in mice with targeted disruption of the HMGI-C locus, and lipomagenesis in humans is frequently associated with somatic mutations of HMGI genes. The aim of this study was to examine the role of HMGI(Y) proteins in adipocytic cell growth and differentiation. First, we found that differentiation of the preadipocytic 3T3-L1 cell line caused early induction of HMGI(Y) gene expression. Suppression of HMGI(Y) expression by antisense technology dramatically increased the growth rate and impaired adipocytic differentiation in these cells. The process of adipogenic differentiation involves the interplay of several transcription factors, among which is the CCAAT/enhancer-binding protein (C/EBP) family of proteins. These factors are required for the transcriptional activation of adipocyte-specific genes. We also tested the hypothesis that HMGI(Y) might participate in transcriptional control of adipocyte-specific promoters. We found that HMGI(Y) proteins bind C/EBPbeta in vivo and in vitro. Furthermore, we show that HMGI(Y) strongly potentiates the capacity of C/EBPbeta to transactivate the leptin promoter, an adipose-specific promoter. Taken together, these results indicate that the HMGI(Y) proteins play a critical role in adipocytic cell growth and differentiation. (literal)
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