http://www.cnr.it/ontology/cnr/individuo/prodotto/ID20839
The soluble ectodomain of RetC634Y inhibits both the wild-type and the constitutively active Ret (Articolo in rivista)
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- Label
- The soluble ectodomain of RetC634Y inhibits both the wild-type and the constitutively active Ret (Articolo in rivista) (literal)
- Anno
- 2003-01-01T00:00:00+01:00 (literal)
- Alternative label
Cerchia L. 1, Libri D. 2, Carlomagno M.S. 3, and De Franciscis V. 1 (2003)
The soluble ectodomain of RetC634Y inhibits both the wild-type and the constitutively active Ret
in Biochemical journal (Lond., 1984)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Cerchia L. 1, Libri D. 2, Carlomagno M.S. 3, and De Franciscis V. 1 (literal)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
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- ISI Web of Science (WOS) (literal)
- Titolo
- The soluble ectodomain of RetC634Y inhibits both the wild-type and the constitutively active Ret (literal)
- Abstract
- Substitution of Cys-634 in the extracellular domain of the Ret
tyrosine kinase receptor causes its dimerization and activation
of its transforming potential. To gain further insight into the
molecular basis leading to Ret activation we purified a mutant
protein consisting of the entire ectodomain of the Ret carrying
a Cys-634?¨Tyr substitution (EC-RetC634Y). The protein is
glycosylated, like the native one, and is biologically active.
By using an in vitro cell system we show that EC-RetC634Y
inhibits the membrane-bound receptor RetC634Y, interfering with
its dimerization. Furthermore, we demonstrate that EC-RetC634Y
competes with the wild-type Ret receptor for ligand binding. The
results presented support the notion of the possible involvment of
glial cell line-derived neurotrophic factor (GDNF) with multiple
endocrine neoplasia type 2A (MEN2A) tumours, and describe a
useful tool for generating molecular mimetics directed towards
specific mutations of the ret oncogene. (literal)
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