Water influx and cell swelling after nanosecond electropermeabilization (Articolo in rivista)

Type
Label
  • Water influx and cell swelling after nanosecond electropermeabilization (Articolo in rivista) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.bbamem.2013.03.007 (literal)
Alternative label
  • Stefania Romeo, Yu-Hsuan Wu, Zachary A. Levine, Martin A. Gundersen, P. Thomas Vernier (2013)
    Water influx and cell swelling after nanosecond electropermeabilization
    in Biochimica et biophysica acta. Biomembranes
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Stefania Romeo, Yu-Hsuan Wu, Zachary A. Levine, Martin A. Gundersen, P. Thomas Vernier (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • CNR - Institute for Electromagnetic Sensing of Environment (IREA), via Diocleziano 328, 80124 - Naples, Italy; Department of Information Engineering, Second University of Naples, via Roma 29, 81031 - Aversa, Italy; Mork Family Department of Chemical Engineering and Material Science, Viterbi School of Engineering, University of Southern California, Los Angeles CA, 90089; Department of Physics and Astronomy, Dornsife College of Letters, Arts, and Sciences, University of Southern California, Los Angeles, CA 90089; Ming Hsieh Department of Electrical Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles CA, 90089 (literal)
Titolo
  • Water influx and cell swelling after nanosecond electropermeabilization (literal)
Abstract
  • Pulsed electric fields are used to permeabilize cell membranes in biotechnology and the clinic. Although molecular and continuum models provide compelling representations of the mechanisms underlying this phenomenon, a clear structural link between the biomolecular transformations displayed in molecular dynamics (MD) simulations and the micro- and macroscale cellular responses observed in the laboratory has not been established. In this paper, plasma membrane electropermeabilization is characterized by exposing Jurkat T lymphoblasts to pulsed electric fields less than 10 ns long (including single pulse exposures), and by monitoring the resulting osmotically driven cell swelling as a function of pulse number and pulse repetition rate. In this way, we reduce the complexity of the experimental system and lay a foundation for gauging the correspondence between measured and simulated values for water and ion transport through electropermeabilized membranes. We find that a single 10 MV/m pulse of 5 ns duration produces measurable swelling of Jurkat T lymphoblasts in growth medium, and we estimate from the swelling kinetics the ion and water flux that follows the electropermeabilization of the membrane. From these observations we set boundaries on the net conductance of the permeabilized membrane, and we show how this is consistent with model predictions for the conductance and areal density of nanoelectropulse-induced lipid nanopores. (literal)
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