The cannabinoid TRPA1 agonist cannabichromene inhibits nitric oxide production in macrophages and ameliorates murine colitis. (Articolo in rivista)

Type
Label
  • The cannabinoid TRPA1 agonist cannabichromene inhibits nitric oxide production in macrophages and ameliorates murine colitis. (Articolo in rivista) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1111/bph.12120 (literal)
Alternative label
  • Romano B 1, Borrelli F 1, Fasolino I 1, Capasso R 1, Piscitelli F 3, Cascio M 4, Pertwee R 4, Coppola D 5, Vassallo L 5, Orlando P 6, Di Marzo V 3, Izzo A 1 (2013)
    The cannabinoid TRPA1 agonist cannabichromene inhibits nitric oxide production in macrophages and ameliorates murine colitis.
    in British journal of pharmacology (Online)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Romano B 1, Borrelli F 1, Fasolino I 1, Capasso R 1, Piscitelli F 3, Cascio M 4, Pertwee R 4, Coppola D 5, Vassallo L 5, Orlando P 6, Di Marzo V 3, Izzo A 1 (literal)
Pagina inizio
  • 213 (literal)
Pagina fine
  • 229 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 169 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 1 (literal)
Note
  • PubMe (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1 Department of Experimental Pharmacology, University of Naples Federico II, Naples, Italy 2 Endocannabinoid Research Group 3 Institute of Biomolecular Chemistry, National Research Council, Pozzuoli, (NA), (Italy) 4 School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK 5 Department of Diagnostic Services (Anatomy and Pathologic Histology Service), Ospedale dei Pellegrini, ASL 1, Naples, Italy 6 Institute of Protein Biochemistry, National Research Council, Naples, Italy (literal)
Titolo
  • The cannabinoid TRPA1 agonist cannabichromene inhibits nitric oxide production in macrophages and ameliorates murine colitis. (literal)
Abstract
  • The non-psychotropic cannabinoid cannabichromene is known to activate the transient receptor potential ankyrin-type1 (TRPA1) and to inhibit endocannabinoid inactivation, both of which are involved in inflammatory processes. We examined here the effects of this phytocannabinoid on peritoneal macrophages and its efficacy in an experimental model of colitis. EXPERIMENTAL APPROACH: Murine peritoneal macrophages were activated in vitro by lipopolysaccharide (LPS). Nitrite levels were measured using a fluorescent assay; inducible nitric oxide (iNOS), cyclooxygenase-2 (COX-2), TRPA1 and cannabinoid (CB(1) and CB(2) ) receptors were analysed by RT-PCR (and/or western blot analysis); colitis was induced by dinitrobenzensulphonic acid (DNBS). Endocannabinoid (anandamide and 2-arachidonoylglycerol), palmitoylethanolamide and oleoylethanolamide levels were measured by liquid chromatography-mass spectrometry. Colonic inflammation was assessed by evaluating the myeloperoxidase activity as well as by histology and immunohistochemistry. caused a significant production of nitrites, associated to up-regulation of anandamide, iNOS, COX-2, CB(1) receptors and down-regulation of CB(2) receptors. mRNA expression. Cannabichromene significantly reduced LPS-stimulated nitrite levels, and its effect was mimicked by cannabinoid receptor and TRPA1 agonists (carvacrol and cinnamaldehyde) and enhanced by CB(1) receptor antagonists. LPS-induced anandamide, iNOS, COX-2, cannabinoid receptor and TRPA1 changes were not significantly modified by cannabichromene, which, however, increased oleoylethanolamide levels. In vivo, cannabichromene ameliorated DNBS-induced colonic inflammation, as revealed by histology, immunohistochemistry, and myeloperoxidase activity. CONCLUSION AND IMPLICATIONS: Cannabichromene exerts anti-inflammatory actions in activated macrophages - with tonic CB(1) cannabinoid signalling being negatively coupled to this effect - and ameliorates experimental murine colitis. (literal)
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