Weak hydrostatic forces in far-scanning ion conductance microscopy used to guide neuronal growth cones (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Weak hydrostatic forces in far-scanning ion conductance microscopy used to guide neuronal growth cones (Articolo in rivista) (literal)

Anno

• 2011-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.neures.2010.11.009 (literal)

Alternative label

• Pellegrino, Mario [ 1 ] ; Orsini Paolo [ 1 ] ; Pellegrini Monica [ 2 ] ; Baschieri Paolo [ 3 ] ; Dinelli Franco [ 3 ] ; Petracchi Donatella [ 3 ] ; Tognoni Elisabetta [ 3 ] ; Ascoli Cesare [ 3 ] (2011)
  Weak hydrostatic forces in far-scanning ion conductance microscopy used to guide neuronal growth cones
  in Neuroscience research (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Pellegrino, Mario [ 1 ] ; Orsini Paolo [ 1 ] ; Pellegrini Monica [ 2 ] ; Baschieri Paolo [ 3 ] ; Dinelli Franco [ 3 ] ; Petracchi Donatella [ 3 ] ; Tognoni Elisabetta [ 3 ] ; Ascoli Cesare [ 3 ] (literal)

Pagina inizio

• 234 (literal)

Pagina fine

• 240 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 69 (literal)

Rivista

• Neuroscience research (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 3 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• [ 1 ] Univ Pisa, Dipartimento Sci Fisiol, I-56127 Pisa, Italy [ 2 ] Scuola Normale Super Pisa, Pisa, Italy [ 3 ] CNR Pisa, Ist Nazl Ott, Pisa, Italy (literal)

Titolo

• Weak hydrostatic forces in far-scanning ion conductance microscopy used to guide neuronal growth cones (literal)

Abstract

• Scanning ion conductance microscopy (SICM) is currently used for high resolution topographic imaging of living cells. Recently, it has been also employed as a tool to deliver stimuli to the cells. In this work we have investigated the mechanical interaction occurring between the pipette tip and the sample during SICM operation. For the purpose, we have built a setup combining SICM with atomic force microscopy (AFM), where the AFM cantilever replaces the sample. Our data indicate that, operating in far-scanning mode with current decrease values below 2%, no force can be detected, provided that the level of the electrolyte filling the pipette is equal to that determined by the capillary tension. A filling level different from this value determines a hydrostatic pressure, a flux through the pipette tip and detectable forces, even in far-scanning mode. The absolute value of these forces depends on the pipette tip size. Therefore, a possible pitfall when using SICM for cell imaging is to imply zero-force working conditions. However the hydrostatic forces can be exploited in order to deliver weak mechanical stimuli and guide neuronal growth cones. Evidences of the effectiveness of this approach are herein given. (C) 2010 Elsevier Ireland Ltd and the Japan Neuroscience Society. (literal)

Prodotto di

• Microscopia Confocale e a Sonda (MD.P03.022.003) (Modulo)

Autore CNR

• FRANCO DINELLI (Unità di personale interno)
• ELISABETTA TOGNONI (Unità di personale interno)
• CESARE ASCOLI (Persona)
• PAOLO BASCHIERI (Unità di personale interno)
• DONATELLA PETRACCHI (Unità di personale esterno)

Insieme di parole chiave

• Parole chiave di "Weak hydrostatic forces in far-scanning ion conductance microscopy used to guide neuronal growth cones" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• FRANCO DINELLI (Unità di personale interno)
• PAOLO BASCHIERI (Unità di personale interno)
• ELISABETTA TOGNONI (Unità di personale interno)
• DONATELLA PETRACCHI (Unità di personale esterno)
• CESARE ASCOLI (Persona)

Prodotto

• Microscopia Confocale e a Sonda (MD.P03.022.003) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Neuroscience research (Rivista)

Insieme di parole chiave di

• Parole chiave di "Weak hydrostatic forces in far-scanning ion conductance microscopy used to guide neuronal growth cones" (Insieme di parole chiave)