AUTOCOUNTER, an ImageJ JavaScript to analyze LC3B-GFP expression dynamics in autophagy-induced astrocytoma cells. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• AUTOCOUNTER, an ImageJ JavaScript to analyze LC3B-GFP expression dynamics in autophagy-induced astrocytoma cells. (Articolo in rivista) (literal)

Anno

• 2012-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.4081/ejh.2012.e44 (literal)

Alternative label

• Fassina L, Magenes G, Inzaghi A, Palumbo S, Allavena G, Miracco C, Pirtoli L, Biggiogera M, Comincini S. (2012)
  AUTOCOUNTER, an ImageJ JavaScript to analyze LC3B-GFP expression dynamics in autophagy-induced astrocytoma cells.
  in European journal of histochemistry (Testo stamp.)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Fassina L, Magenes G, Inzaghi A, Palumbo S, Allavena G, Miracco C, Pirtoli L, Biggiogera M, Comincini S. (literal)

Pagina inizio

• e44 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 56(4) (literal)

Rivista

• European journal of histochemistry (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Department of Industrial Engineering and Information, University of Pavia; Centre for Tissue Engineering (C.I.T.), University of Pavia; Department of Biology and Biotechnology \"Lazzaro Spallanzani\", University of Pavia;; Department of Human Pathology and Oncology, University of Siena, Italy (literal)

Titolo

• AUTOCOUNTER, an ImageJ JavaScript to analyze LC3B-GFP expression dynamics in autophagy-induced astrocytoma cells. (literal)

Abstract

• An ImageJ JavaScript, AUTOCOUNTER, was specifically developed to monitor and measure LC3B-GFP expression in living human astrocytoma cells, namely T98G and U373-MG. Discrete intracellular GFP fluorescent spots derived from transduction of a Baculovirus replication-defective vector (BacMam LC3B-GFP), followed by microscope examinations at different times. After viral transgene expression, autophagy was induced by Rapamycin administration and assayed in ph-p70S6K/p70S6K and LC3B immunoblotting expression as well as by electron microscopy examinations. A mutated transgene, defective in LC3B lipidation, was employed as a negative control to further exclude fluorescent dots derived from protein intracellular aggregation. The ImageJ JavaScript was then employed to evaluate and score the dynamics changes of the number and area of LC3B-GFP puncta per cell in time course assays and in complex microscope examinations. In conclusion, AUTOCOUNTER enabled to quantify LC3B-GFP expression and to monitor dynamics changes in number and shapes of autophagosomal-like vesicles: it might therefore represent a suitable algorithmic tool for in vitro autophagy modulation studies. (literal)

Prodotto di

• Institute of molecular genetics (IGM) (Istituto)
• Sviluppo di nuove tecniche e metodologie ottiche per biologia di base e applicazioni biomediche (ME.P04.004.002) (Modulo)

Autore CNR

• Marco Biggiogera (Persona)

Incoming links:

Prodotto

• Institute of molecular genetics (IGM) (Istituto)
• Sviluppo di nuove tecniche e metodologie ottiche per biologia di base e applicazioni biomediche (ME.P04.004.002) (Modulo)

Autore CNR di

• Marco Biggiogera (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• European journal of histochemistry (Rivista)