Alternative splicing of the human estrogen receptor \alpha primary transcript: Mechanisms of exon skipping (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Alternative splicing of the human estrogen receptor \alpha primary transcript: Mechanisms of exon skipping (Articolo in rivista) (literal)

Anno

• 2003-01-01T00:00:00+01:00 (literal)

Alternative label

• P. Ferro, A. Forlani, M. Muselli, U. Pfeffer (2003)
  Alternative splicing of the human estrogen receptor \alpha primary transcript: Mechanisms of exon skipping
  in International Journal of Molecular Medicine
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• P. Ferro, A. Forlani, M. Muselli, U. Pfeffer (literal)

Pagina inizio

• 355 (literal)

Pagina fine

• 363 (literal)

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• 12 (literal)

Rivista

• International Journal of Molecular Medicine (Rivista)

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• 9 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 3 (literal)

Note

• PubMe (literal)
• Scopu (literal)
• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• P. Ferro: Laboratory of Molecular Oncology, National Cancer Research Institute, I-16132 Genova, Italy; A. Forlani: Laboratory of Molecular Oncology, National Cancer Research Institute, I-16132 Genova, Italy; M. Muselli: CNR-IEIIT, Genova, Italy; U. Pfeffer: Laboratory of Molecular Oncology, National Cancer Research Institute, I-16132 Genova, Italy. (literal)

Titolo

• Alternative splicing of the human estrogen receptor \alpha primary transcript: Mechanisms of exon skipping (literal)

Abstract

• The 1785 nucleotides of the coding region of the estrogen receptor \alpha (ER-\alpha) are dispersed over a region of more than 300.000 nucleotides in the primary transcript. Splicing of this precursor RNA frequently leads to variants lacking one or more exons that have been associated to breast cancer progression. The most frequent splice variant lacks exon 4 and is expressed in the human mammary carcinoma cell line MCF-7 at a level similar to that of the full-length messenger. The in silico analysis of ER-\alpha splice sites by Hamming clustering, a self learning method trained on more than 28.000 experimentally proved splice sites, reveals high relevance for the 5' and 3' splice sites of exon 4. The splicing analysis of transfected mini-gene constructs containing drastically shortened introns excludes that weak splice sites, intron or exon lengths or splice enhancers are responsible for exon skipping. Exon 6 is never skipped in MCF-7 cells but is spliced out from mine-gene derived primary transcripts if inserted between exons 3 and 5 instead of exon 4. As a consequence, it appears that a particular splice site affinity of exon 3 donor and exon 5 acceptor sites is responsible for skipping of the exon in between. (literal)

Prodotto di

• Istituto di elettronica e di ingegneria dell'informazione e delle telecomunicazioni (IEIIT) (Istituto)
• Machine Learning for Biological Data (INT.P02.002.001) (Modulo)

Autore CNR

• MARCO MUSELLI (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Alternative splicing of the human estrogen receptor \alpha primary transcript: Mechanisms of exon skipping" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di elettronica e di ingegneria dell'informazione e delle telecomunicazioni (IEIIT) (Istituto)
• Machine Learning for Biological Data (INT.P02.002.001) (Modulo)

Autore CNR di

• MARCO MUSELLI (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• International Journal of Molecular Medicine (Rivista)

Insieme di parole chiave di

• Keywords of "Alternative splicing of the human estrogen receptor \alpha primary transcript: Mechanisms of exon skipping" (Insieme di parole chiave)