PROBING PROTEIN-STRUCTURE BY SOLVENT PERTURBATION OF NMR-SPECTRA - A COMPARISON WITH PHOTOCHEMICALLY INDUCED DYNAMIC NUCLEAR-POLARIZATION TECHNIQUES APPLIED TO NATIVE ALPHA-LACTALBUMIN (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• PROBING PROTEIN-STRUCTURE BY SOLVENT PERTURBATION OF NMR-SPECTRA - A COMPARISON WITH PHOTOCHEMICALLY INDUCED DYNAMIC NUCLEAR-POLARIZATION TECHNIQUES APPLIED TO NATIVE ALPHA-LACTALBUMIN (Articolo in rivista) (literal)

Anno

• 1995-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1111/j.1432-1033.1995.tb20361.x (literal)

Alternative label

• IMPROTA, S; MOLINARI, H; PASTORE, A; CONSONNI, R; ZETTA, L (1995)
  PROBING PROTEIN-STRUCTURE BY SOLVENT PERTURBATION OF NMR-SPECTRA - A COMPARISON WITH PHOTOCHEMICALLY INDUCED DYNAMIC NUCLEAR-POLARIZATION TECHNIQUES APPLIED TO NATIVE ALPHA-LACTALBUMIN
  in European journal of biochemistry (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• IMPROTA, S; MOLINARI, H; PASTORE, A; CONSONNI, R; ZETTA, L (literal)

Pagina inizio

• 78 (literal)

Pagina fine

• 86 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1995.tb20361.x/pdf (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 227 (literal)

Rivista

• European journal of biochemistry (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• CONSONNI, R; ZETTA, L ISMAC Milano (literal)

Titolo

• PROBING PROTEIN-STRUCTURE BY SOLVENT PERTURBATION OF NMR-SPECTRA - A COMPARISON WITH PHOTOCHEMICALLY INDUCED DYNAMIC NUCLEAR-POLARIZATION TECHNIQUES APPLIED TO NATIVE ALPHA-LACTALBUMIN (literal)

Abstract

• We have suggested elsewhere the use of surface mapping by spin label probes (Esposito et al., 1992). According to this approach, soluble nitroxides are added to a protein solution. Resonances of protons that are accessible to the nitroxide are broadened and bleached out of the spectrum, while resonances in the protein interior remain unaffected. This approach is, in principle, complementary to another technique, photochemically induced dynamic nuclear polarization, which maps the position of aromatic protons on the protein surface. A detailed comparison between the two techniques is necessary for a confident use of the more recent suggested nitroxide perturbation approach. In the present study, we show that the results obtained by the two techniques for the native state of bovine alpha-lactalbumin are fully consistent and may therefore be combined for the study of protein surfaces. (literal)

Prodotto di

• Studio di metaboliti e matrici naturali mediante NMR e loro utilizzo e funzione nel settore agroalimentare, merceologico e della salute umana (PM.P06.018.001) (Modulo)
• Istituto per lo studio delle macromolecole (ISMAC) (Istituto)

Autore CNR

• ROBERTO CONSONNI (Unità di personale interno)
• LUCIA ZETTA (Persona)

Insieme di parole chiave

• Keywords of "PROBING PROTEIN-STRUCTURE BY SOLVENT PERTURBATION OF NMR-SPECTRA - A COMPARISON WITH PHOTOCHEMICALLY INDUCED DYNAMIC NUCLEAR-POLARIZATION TECHNIQUES APPLIED TO NATIVE ALPHA-LACTALBUMIN" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• ROBERTO CONSONNI (Unità di personale interno)
• LUCIA ZETTA (Persona)

Prodotto

• Istituto per lo studio delle macromolecole (ISMAC) (Istituto)
• Studio di metaboliti e matrici naturali mediante NMR e loro utilizzo e funzione nel settore agroalimentare, merceologico e della salute umana (PM.P06.018.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• European journal of biochemistry (Rivista)

Insieme di parole chiave di

• Keywords of "PROBING PROTEIN-STRUCTURE BY SOLVENT PERTURBATION OF NMR-SPECTRA - A COMPARISON WITH PHOTOCHEMICALLY INDUCED DYNAMIC NUCLEAR-POLARIZATION TECHNIQUES APPLIED TO NATIVE ALPHA-LACTALBUMIN" (Insieme di parole chiave)