NEW TYPES OF LARGE-PORE POLYACRYLAMIDE-AGAROSE MIXED-BED MATRICES FOR DNA ELECTROPHORESIS - PORE-SIZE ESTIMATION FROM FERGUSON PLOTS OF DNA FRAGMENTS (Articolo in rivista)

Type
Label
  • NEW TYPES OF LARGE-PORE POLYACRYLAMIDE-AGAROSE MIXED-BED MATRICES FOR DNA ELECTROPHORESIS - PORE-SIZE ESTIMATION FROM FERGUSON PLOTS OF DNA FRAGMENTS (Articolo in rivista) (literal)
Anno
  • 1995-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1002/elps.11501601221 (literal)
Alternative label
  • CHIARI, M; DALESIO, L; CONSONNI, R; RIGHETTI, PG (1995)
    NEW TYPES OF LARGE-PORE POLYACRYLAMIDE-AGAROSE MIXED-BED MATRICES FOR DNA ELECTROPHORESIS - PORE-SIZE ESTIMATION FROM FERGUSON PLOTS OF DNA FRAGMENTS
    in Electrophoresis (Weinh., Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • CHIARI, M; DALESIO, L; CONSONNI, R; RIGHETTI, PG (literal)
Pagina inizio
  • 1337 (literal)
Pagina fine
  • 1344 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://onlinelibrary.wiley.com/doi/10.1002/elps.11501601221/pdf (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 16 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • CHIARI, M ICRM MIlano CONSONNI, R ISMAC Milano (literal)
Titolo
  • NEW TYPES OF LARGE-PORE POLYACRYLAMIDE-AGAROSE MIXED-BED MATRICES FOR DNA ELECTROPHORESIS - PORE-SIZE ESTIMATION FROM FERGUSON PLOTS OF DNA FRAGMENTS (literal)
Abstract
  • The average pore size value of gels containing polyacrylamide, covalently linked to agarose, was found to be 30% higher than the value of a regular N,N'-methylenebisacrylamide (Bis) cross-linked gel of the same %T. By increasing the agarose concentration (10% of the total amount of polyacrylamide), gels containing low amounts of acrylamide (1.5-2%) are reproducibly obtained; their pore sizes are 130% larger than the pore sizes of a 4%T, 3.3%C polyacrylamide gel. In general, mixed-bed matrices were found to be more elastic and mechanically stronger than classical polyacrylamide gels since an agarose-induced gelation process takes place during their polymerization. (literal)
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