Identification and characterization of PlAlix, the Alix homologue from the Mediterranean sea urchin Paracentrotus lividus (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Identification and characterization of PlAlix, the Alix homologue from the Mediterranean sea urchin Paracentrotus lividus (Articolo in rivista) (literal)

Anno

• 2013-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1111/dgd.12023 (literal)

Alternative label

• Romancino D.P., , Anello L., Morici G. d'Azzò A., ,.Bongiovanni A. ,.DiBernardo M. (2013)
  Identification and characterization of PlAlix, the Alix homologue from the Mediterranean sea urchin Paracentrotus lividus
  in Development, growth & differentiation (Online); Japanese society of developmental biologists, Hyogo (Giappone)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Romancino D.P., , Anello L., Morici G. d'Azzò A., ,.Bongiovanni A. ,.DiBernardo M. (literal)

Pagina inizio

• 237 (literal)

Pagina fine

• 246 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 55 (literal)

Rivista

• Development, growth & differentiation (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 10 (literal)

Note

• Scopus (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• IBIM-CNR Dipartimento di Scienze e Tecnologie Molecolari e Biomolecolari (STEMBIO), Università di Palermo Department of Genetics, St. Jude Children's Research Hospital,Memphis, TN, USA (literal)

Titolo

• Identification and characterization of PlAlix, the Alix homologue from the Mediterranean sea urchin Paracentrotus lividus (literal)

Abstract

• The sea urchin provides a relatively simple and tractable system for analyzing the early stages of embryo development. Here, we use the sea urchin species, Paracentrotus lividus, to investigate the role of Alix in key stages of embryogenesis, namely the egg fertilization and the first cleavage division. Alix is a multifunctional protein involved in different cellular processes including endocytic membrane trafficking, filamentous (F)-actin remodeling, and cytokinesis. Alix homologues have been identified in different metazoans; in these organisms, Alix is involved in oogenesis and in determination/differentiation events during embryo development. Herein, we describe the identification of the sea urchin homologue of Alix, PlAlix. The deduced amino acid sequence shows that Alix is highly conserved in sea urchins. Accordingly, we detect the PlAlix protein cross-reacting with monoclonal Alix antibodies in extracts from P. lividus, at different developmental stages. Focusing on the role of PlAlix during early embryogenesis we found that PlAlix is a maternal protein that is expressed at increasingly higher levels from fertilization to the 2-cell stage embryo. In sea urchin eggs, PlAlix localizes throughout the cytoplasm with a punctuated pattern and, soon after fertilization, accumulates in larger puncta in the cytosol, and in microvilli- like protrusions. Together our data show that PlAlix is structurally conserved from sea urchin to mammals and may open new lines of inquiry into the role of Alix during the early stages of embryo development. (literal)

Editore

• Japanese society of developmental biologists (Editore)

Prodotto di

• Controllo trascrizionale e post-trascrizionale nello sviluppo, nel differenziamento cellulare e nella trasduzione del segnale (SV.P15.002.001) (Modulo)
• Istituto di biomedicina e di immunologia molecolare \"Alberto Monroy\" (IBIM) (Istituto)

Autore CNR

• MARIA DI BERNARDO (Persona)
• LETIZIA ANELLO (Persona)
• ANTONELLA BONGIOVANNI (Persona)
• DANIELE ROMANCINO (Persona)

Insieme di parole chiave

• Keywords of "Identification and characterization of PlAlix, the Alix homologue from the Mediterranean sea urchin Paracentrotus lividus" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• LETIZIA ANELLO (Persona)
• MARIA DI BERNARDO (Persona)
• DANIELE ROMANCINO (Persona)
• ANTONELLA BONGIOVANNI (Persona)

Prodotto

• Istituto di biomedicina e di immunologia molecolare \"Alberto Monroy\" (IBIM) (Istituto)
• Controllo trascrizionale e post-trascrizionale nello sviluppo, nel differenziamento cellulare e nella trasduzione del segnale (SV.P15.002.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Development, growth & differentiation (Rivista)

Editore di

• Japanese society of developmental biologists (Editore)

Insieme di parole chiave di

• Keywords of "Identification and characterization of PlAlix, the Alix homologue from the Mediterranean sea urchin Paracentrotus lividus" (Insieme di parole chiave)