Capsaicin-induced corneal sensory denervation and healing impairment are reversed by NGF treatment (Articolo in rivista)

Type
Label
  • Capsaicin-induced corneal sensory denervation and healing impairment are reversed by NGF treatment (Articolo in rivista) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1167/iovs.12-10593 (literal)
Alternative label
  • Lambiase Alessandro1; Aloe Luigi2; Mantelli Flavio1; Sacchetti Marta1; Perrella Eleonora3; Bianchi Patrizia2; Rocco Maria Luisa2; Bonini Stefano1 (2012)
    Capsaicin-induced corneal sensory denervation and healing impairment are reversed by NGF treatment
    in Investigative Ophtalmology and Visual Science
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Lambiase Alessandro1; Aloe Luigi2; Mantelli Flavio1; Sacchetti Marta1; Perrella Eleonora3; Bianchi Patrizia2; Rocco Maria Luisa2; Bonini Stefano1 (literal)
Pagina inizio
  • 8280 (literal)
Pagina fine
  • 8287 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 53 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 13 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Departments of 1 Ophthalmology and 3 Pathology, Campus Bio-Medico University of Rome, Italy. 2 Institute of Neurobiology and Molecular Medicine (CNR), Rome, Rome, Italy(*) (*) Confluito nell'IBCN con provvedimento del Presidente del CNR n. 116 del 21/12/2010 prot. n. 0091899 (literal)
Titolo
  • Capsaicin-induced corneal sensory denervation and healing impairment are reversed by NGF treatment (literal)
Abstract
  • PURPOSE: We aimed to evaluate the nerve growth factor (NGF) pathway and its influence on corneal healing mechanisms in normal conditions and in an animal model of corneal denervation induced by capsaicin. METHODS: Peripheral sensory damage was induced in rat pups by subcutaneous injection of capsaicin and the effects evaluated by hot-plate test, corneal nerve count, and tear secretion. Corneal damage was induced in capsaicin-treated and -untreated rats by epithelial scraping. Healing rate; NGF pathway (NGF, tyrosine kinase A [TrkA], p75); and the stem cell marker p63 were evaluated by RT-PCR, ELISA, Western blot, and immunohistochemistry. The effects of exogenous NGF administration as eye drop formulation were also tested. RESULTS: Capsaicin treatment induced a significant reduction of peripheral sensitivity, corneal innervation, tear secretion, and corneal healing rate. The ocular effects of capsaicin treatment were associated with an NGF pathway alteration. NGF eye drop treatment aided corneal healing mechanisms through a significant increase in the NGF receptors TrkA and p75, and in the stem cell marker p63. CONCLUSIONS: In this study, we show that an alteration in the NGF pathway is responsible for a delay in corneal healing in an animal model of sensory denervation. Moreover, we show that NGF eye drop administration modulates corneal innervation, epithelial cell healing, and corneal stem cells. These findings may trigger further research on the role of the NGF pathway in limbal stem cell deficiency. (literal)
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