Correlative light-electron microscopy as a tool to study in vivo dynamics and ultrastructure of intracellular structures (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Correlative light-electron microscopy as a tool to study in vivo dynamics and ultrastructure of intracellular structures (Articolo in rivista) (literal)

Anno

• 2013-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1007/978-1-62703-056-4_20 (literal)

Alternative label

• Polishchuk EV, Polishchuk RS, Luini A. (2013)
  Correlative light-electron microscopy as a tool to study in vivo dynamics and ultrastructure of intracellular structures
  in Methods in molecular biology (Clifton N.J.)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Polishchuk EV, Polishchuk RS, Luini A. (literal)

Pagina inizio

• 413 (literal)

Pagina fine

• 422 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://link.springer.com/protocol/10.1007%2F978-1-62703-056-4_20 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 931 (literal)

Rivista

• Methods in molecular biology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Institute of Protein Biochemistry, Naples, Italy. (literal)

Titolo

• Correlative light-electron microscopy as a tool to study in vivo dynamics and ultrastructure of intracellular structures (literal)

Abstract

• Correlative light-electron microscopy (CLEM) is a very effective technique that combines live-cell imaging and immuno-electron microscopy for ultrastructural morphological characterization of dynamic intracellular organelles. The use of green fluorescent protein (GFP)-tagged chimeras allows the user to follow the movements and/or behavior of intracellular structures in a live cell and to fix it at the moment of interest. The subsequent immuno-electron microscopy processing can then reveal the three-dimensional architecture of the same structure, together with precise recognition of the GFP-labeled protein. The process resembles the taking of a high-resolution snapshot of an interesting live scene. Considering that CLEM is a very useful but technically demanding and time-consuming technique, accurate protocols will be helpful to simplify the work of scientists who are willing to apply this method for their own purposes. Here, we present a detailed protocol that describes all of the \"tricks\" and know-hows involved in carrying out the crucial steps of a CLEM experiment. (literal)

Prodotto di

• Dr.ssa CORDA Meccanismi molecolari coinvolti nella trasformazione neoplastica: identificazione di bersagli specifici per lo sviluppo di nuovi farmaci (SV.P15.014.001) (Modulo)
• Istituto di biochimica delle proteine (IBP) (Istituto)

Autore CNR

• ALBERTO LUINI (Persona)

Incoming links:

Prodotto

• Istituto di biochimica delle proteine (IBP) (Istituto)
• Dr.ssa CORDA Meccanismi molecolari coinvolti nella trasformazione neoplastica: identificazione di bersagli specifici per lo sviluppo di nuovi farmaci (SV.P15.014.001) (Modulo)

Autore CNR di

• ALBERTO LUINI (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Methods in molecular biology (Rivista)