http://www.cnr.it/ontology/cnr/individuo/prodotto/ID201040

Detection of the BRAFV600E Mutation in Fine Needle Aspiration Cytology of Thyroid Papillary Microcarcinoma Cells Selected by Manual Macrodissection: An Easy Tool to Improve the Preoperative Diagnosis (Articolo in rivista)

Type

Articolo in rivista (Classe)
Prodotto della ricerca (Classe)

Label

Detection of the BRAFV600E Mutation in Fine Needle Aspiration Cytology of Thyroid Papillary Microcarcinoma Cells Selected by Manual Macrodissection: An Easy Tool to Improve the Preoperative Diagnosis (Articolo in rivista) (literal)

Anno

2012-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1089/thy.2011.0107 (literal)

Alternative label

Marchetti, Ivo (3); Iervasi, Giorgio (1); Mazzanti, Chiara Maria (4); Lessi, Francesca (4); Tomei, Sara (4); Naccarato, Antonio Giuseppe (5); Aretini, Paolo (5); Alberti, Baldassarre (2); Di Coscio, Giancarlo (3); Bevilacqua, Generoso (5) (2012)
Detection of the BRAFV600E Mutation in Fine Needle Aspiration Cytology of Thyroid Papillary Microcarcinoma Cells Selected by Manual Macrodissection: An Easy Tool to Improve the Preoperative Diagnosis
in Thyroid (N.Y.N.Y.); Mary Ann Liebert Inc., New Rochelle (Stati Uniti d'America)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Marchetti, Ivo (3); Iervasi, Giorgio (1); Mazzanti, Chiara Maria (4); Lessi, Francesca (4); Tomei, Sara (4); Naccarato, Antonio Giuseppe (5); Aretini, Paolo (5); Alberti, Baldassarre (2); Di Coscio, Giancarlo (3); Bevilacqua, Generoso (5) (literal)

Pagina inizio

292 (literal)

Pagina fine

298 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

ID_PUMA: cnr.ifc/2011-A0-092. - Online Ahead of Print: December 19, 2011 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

22 (literal)

Rivista

Thyroid (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

3 (literal)

Note

ISI Web of Science (WOS) (literal)
Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

(1) CNR-IFC, Pisa; (2) Fondazione G. Monasterio-Regione Toscana/CNR and Institute of Clinical Physiology; (3) Section of Cytopathology, University of Pisa and Pisa University Hospital, Pisa, Italy.; (4) Section of Molecular Pathology and 4Division of Surgical, Molecular, and Ultrastructural Pathology; University of Pisa and Pisa University Hospital, Pisa, Italy; (5) Division of Surgical, Molecular, and Ultrastructural Pathology; University of Pisa and Pisa University Hospital, Pisa, Italy (literal)

Titolo

Detection of the BRAFV600E Mutation in Fine Needle Aspiration Cytology of Thyroid Papillary Microcarcinoma Cells Selected by Manual Macrodissection: An Easy Tool to Improve the Preoperative Diagnosis (literal)

Abstract

Background: Papillary carcinomas with diameters that are less than or equal to 1 cm (thyroid papillary microcarcinoma [mPTC]) are quite common but can carry more risk than previously thought. The proper treatment and management of these lesions is still being debated. Even though fine needle aspiration cytology (FNAC) is considered the best method for the diagnosis of thyroid nodules, its efficacy is still questioned for mPTC. We investigated the role of BRAF gene status in preoperative cytological samples, using manual macrodissection as an additional tool to improve the diagnostic accuracy of mPTC. Methods: DNA was extracted directly from stained FNAC smears of 95 patients including 85 with histological diagnoses of papillary thyroid carcinoma (PTC) £ 1 cm and 10 with goiters. The cytological diagnoses of the 95 cases included the following: 42 samples were suspicious for papillary carcinoma, 38 were PTCs, and 15 were indeterminate lesions. DNA was then extracted from the FNAC slides after performing a ''manual macrodissection'' procedure. The BRAFV600E mutational status was determined by sequence analysis in all the patients. Results: In this study, we showed that the BRAFV600E mutation was present with a high frequency in patients with mPTC (74%). The presence of the mutation was independent of the size of the tumor. In our study, the combination of the cytological diagnosis and the molecular analysis was able to identify 82% of all cases of mPTC, with an increase of 37% compared with a morphological diagnosis alone. The morpho-molecular analysis was able to reduce the number of suspicious cases by > 70%. All of the goiters had a wild-type BRAF status. Conclusions: The analysis of BRAF mutational status in FNAC obtained from papillary microcarcinomas demonstrates thatmolecular pathology, combined withmorphology andmolecular biology is a powerful tool for cytological diagnosis ofmPTC.Our results also confirm the data supporting the biological relevance of PTCswith diameters that are £ 1 cm and the importance of ''manual macrodissection'' in the molecular analysis of cytological material. (literal)

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