http://www.cnr.it/ontology/cnr/individuo/prodotto/ID200896
Identification of a single-stranded DNA virus associated with citrus chlorotic dwarf disease, a new member in the family Geminiviridae (Articolo in rivista)
- Type
- Label
- Identification of a single-stranded DNA virus associated with citrus chlorotic dwarf disease, a new member in the family Geminiviridae (Articolo in rivista) (literal)
- Anno
- 2012-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.virol.2012.06.005 (literal)
- Alternative label
Loconsole G., Saldarelli P., Doddapaneni H., Savino V., Martelli G.P., Saponari M. (2012)
Identification of a single-stranded DNA virus associated with citrus chlorotic dwarf disease, a new member in the family Geminiviridae
in Virology (N.Y.N.Y., Print); ELSEVIER, NEW YORK (Stati Uniti d'America)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Loconsole G., Saldarelli P., Doddapaneni H., Savino V., Martelli G.P., Saponari M. (literal)
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- L.G., S.V., M.G.P.: Dipartimento di Biologia e Chimica Agroforestale ed Ambientale, Università degli Studi di Bari, Italy
S.P., S.M.: Istituto di Virologia Vegetale, CNR,UOS Bari, Bari, Italy
D.H.: Carver Center for Genomics, Department of Biology, The University of Iowa, Iowa City, IA 52242, USA (literal)
- Titolo
- Identification of a single-stranded DNA virus associated with citrus chlorotic dwarf disease, a new member in the family Geminiviridae (literal)
- Abstract
- In the attempt to identify the causal agent of Citrus chlorotic dwarf disease (CCDD), a virus-like disorder of citrus, the small RNA fraction and total DNA from symptomatic citrus plants were subjected to high-throughput sequencing. DNA fragments deriving from an apparently new geminivirus-like agent were found and assembled by NGS to re-construct the entire viral genome. The newly identified virus has a circular single-stranded DNA genome comprising five open reading frames (ORFs) with sequence homologies with those encoded by geminiviruses. PCR and qPCR assays were successfully used for determining its presence in the CCDD-affected plants obtained by graft propagation. The larger genome size (3.64 vs. 2.5-3.0 kb) and a number of differences in its structural organization, identified this virus as a highly divergent member of the family Geminiviridae, to which the provisional name of Citrus chlorotic dwarf-associated virus (CCDaV) is assigned. (literal)
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