http://www.cnr.it/ontology/cnr/individuo/prodotto/ID200665

In vitro exposure to nicotine induces endocytosis of presynaptic AMPA receptors modulating dopamine release in rat nucleus accumbens nerve terminals (Articolo in rivista)

Type

Prodotto della ricerca (Classe)
Articolo in rivista (Classe)

Label

In vitro exposure to nicotine induces endocytosis of presynaptic AMPA receptors modulating dopamine release in rat nucleus accumbens nerve terminals (Articolo in rivista) (literal)

Anno

2012-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1016/j.neuropharm.2012.06.049 (literal)

Alternative label

Grilli M; Summa M; Salamone A; Olivero G; Zappettini S; Di Prisco S; Feligioni M; Usai C; Pittaluga A; Marchi M (2012)
In vitro exposure to nicotine induces endocytosis of presynaptic AMPA receptors modulating dopamine release in rat nucleus accumbens nerve terminals
in Neuropharmacology; Pergamon-Elsevier Press Science LDT, Oxford (Regno Unito)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Grilli M; Summa M; Salamone A; Olivero G; Zappettini S; Di Prisco S; Feligioni M; Usai C; Pittaluga A; Marchi M (literal)

Pagina inizio

916 (literal)

Pagina fine

926 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

http://www.sciencedirect.com/science/article/pii/S0028390812003073# (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

63 (literal)

Rivista

Neuropharmacology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

5 (literal)

Note

ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

Univ Genoa, Dept Expt Med, Pharmacol & Toxicol Sect, I-16148 Genoa, Italy; CNR, Inst Biophys, I-16149 Genoa, Italy; Univ Genoa, Ctr Excellence Biomed Res, I-16148 Genoa, Italy; European Brain Res Inst, Pharmacol Synapt Plast Unit, I-00143 Rome, Italy (literal)

Titolo

In vitro exposure to nicotine induces endocytosis of presynaptic AMPA receptors modulating dopamine release in rat nucleus accumbens nerve terminals (literal)

Abstract

Here we provide functional and immunocytochemical evidence supporting the presence on Nucleus Accumbens (NAc) dopaminergic terminals of cyclothiazide-sensitive, alfa-amino-3-hydroxy-5-methyl-4-isoxazolone propionate (AMPA) receptors, which activation causes Ca2+-dependent [3H]dopamine ([3H]DA) exocytosis. These AMPA receptors cross-talk with co-localized nicotinic receptors (nAChRs), as suggested by the finding that in vitro short-term pre-exposure of synaptosomes to 30 ?M nicotine caused a significant reduction of both the 30 ?M nicotine and the 100 ?M AMPA-evoked [3H]DA overflow. Entrapping pep2-SVKI, a peptide known to compete for the binding of GluA2 subunit to scaffolding proteins involved in AMPA receptor endocytosis, in NAC synaptosomes prevented the nicotine-induced reduction of AMPA-mediated [3H]DA exocytosis, while pep2-SVKE, used as negative control, was inefficacious. Immunocytochemical studies showed that a significant percentage of NAc terminals were dopaminergic and that most of these terminals also posses GluA2 receptor subunits. Western blot analysis of GluA2 immunoreactivity showed that presynaptic GluA2 proteins in NAc terminals were reduced in nicotine-pretreated synaptosomes when compared to the control. The nACh-AMPA receptor-receptor interaction was not limited to dopaminergic terminals since nicotine pre-exposure also affected the presynaptic AMPA receptors controlling hippocampal noradrenaline release, but not the presynaptic AMPA receptors controlling GABA and acetylcholine release. These observations could be relevant to the comprehension of the molecular mechanisms at the basis of nicotine rewarding. (literal)

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