http://www.cnr.it/ontology/cnr/individuo/prodotto/ID200449

Knockout of pgdS and ggt genes improves ?-PGA yield in B. subtilis (Articolo in rivista)

Type

Label

Knockout of pgdS and ggt genes improves ?-PGA yield in B. subtilis (Articolo in rivista) (literal)

Anno

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

Alternative label

Scoffone V, Dondi D, Biino G, Borghese G, Pasini D, Galizzi A, Calvio C. (2013)
Knockout of pgdS and ggt genes improves ?-PGA yield in B. subtilis
in Biotechnology and bioengineering (Online); Wiley, New York (Stati Uniti d'America)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Scoffone V, Dondi D, Biino G, Borghese G, Pasini D, Galizzi A, Calvio C. (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

Rivista

Note

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

Dept. of Biology and Biotechnology, Università degli Studi di Pavia, Pavia, Italy. (literal)

Titolo

Abstract

One of the emerging biopolymers that are currently under active investigation is bacterial poly (?-glutamic acid) (?-PGA). However, before its full industrial exploitation, a substantial increase in microbial productivity is required. ?-PGA obtained from the Bacillus subtilis laboratory strain 168 offers the advantage of a producer characterized by a well defined genetic framework and simple manipulation techniques. In this strain, the knockout of genes for the major ?-PGA degrading enzymes, pgdS and ggt, leads to a considerable improvement in polymer yield, which attains levels analogous to the top wild ?-PGA producer strains. This study highlights the convenience of using the laboratory strain of B. subtilis over wild isolates in designing strain improvement strategies aimed at increasing ?-PGA productivity. (literal)

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