Single particle tracking of acyl carrier protein (ACP)-tagged TrkA receptors in PC12nnr5 cells (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Single particle tracking of acyl carrier protein (ACP)-tagged TrkA receptors in PC12nnr5 cells (Articolo in rivista) (literal)

Anno

• 2012-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.jneumeth.2011.10.019 (literal)

Alternative label

• Callegari, A; Luin, S; Marchetti, L; Duci, A; Cattaneo, A; Beltram, F (2012)
  Single particle tracking of acyl carrier protein (ACP)-tagged TrkA receptors in PC12nnr5 cells
  in Journal of neuroscience methods
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Callegari, A; Luin, S; Marchetti, L; Duci, A; Cattaneo, A; Beltram, F (literal)

Pagina inizio

• 82 (literal)

Pagina fine

• 86 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 204 (literal)

Rivista

• Journal of neuroscience methods (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Scuola Normale Super Pisa, NEST Lab, I-56126 Pisa, Italy Ist Nanosc CNR, I-56126 Pisa, Italy Ctr Nanotechnol Innovat, IIT NEST, I-56126 Pisa, Italy (literal)

Titolo

• Single particle tracking of acyl carrier protein (ACP)-tagged TrkA receptors in PC12nnr5 cells (literal)

Abstract

• There is a wide interest in studying the membrane mobility of Nerve Growth Factor (NGF) tropomyosin receptor kinase A (TrkA) at the single molecule level, in order to elucidate its diverse signaling responses related to different receptor functions. Here we present an experimental strategy based on the acyl carrier protein (ACP) tag in order to study the dynamics of the high-affinity NGF receptor TrkA in the membrane of PC12nnr5 cells. We present a single-particle tracking (SPT) study using highly photostable semiconductor quantum dots (Qdots) conjugated to ACP-tagged TrkA receptors. We demonstrate that ACP-TrkA shows biochemical and biological properties identical to those of its unmodified counterpart and that single receptor molecules in living cells display distinct diffusive regimes and a highly heterogeneous dynamics. (C) 2011 Elsevier B.V. All rights reserved. (literal)

Prodotto di

• Progettazione ed applicazioni di Nanobiotecnologie (MD.P06.022.002) (Modulo)

Autore CNR

• STEFANO LUIN (Persona)
• FABIO BELTRAM (Persona)
• ANDREA CALLEGARI (Unità di personale esterno)
• LAURA MARCHETTI (Unità di personale esterno)

Incoming links:

Autore CNR di

• STEFANO LUIN (Persona)
• ANDREA CALLEGARI (Unità di personale esterno)
• FABIO BELTRAM (Persona)
• LAURA MARCHETTI (Unità di personale esterno)

Prodotto

• Progettazione ed applicazioni di Nanobiotecnologie (MD.P06.022.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of neuroscience methods (Rivista)