D-amino acid oxidase from Trigonopsis variabilis: Immobilization of whole cells in natural polymeric gels for glutaryl-7-aminocephalosporanic acid production (Articolo in rivista)

Type
Label
  • D-amino acid oxidase from Trigonopsis variabilis: Immobilization of whole cells in natural polymeric gels for glutaryl-7-aminocephalosporanic acid production (Articolo in rivista) (literal)
Anno
  • 1996-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/0922-338X(96)87591-X (literal)
Alternative label
  • D'Acunzo, A.a, De Alteriis, E.a, Maurano, F.a, Battistel, E.b, Parascandola, P.cd (1996)
    D-amino acid oxidase from Trigonopsis variabilis: Immobilization of whole cells in natural polymeric gels for glutaryl-7-aminocephalosporanic acid production
    in Journal of fermentation and bioengineering (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • D'Acunzo, A.a, De Alteriis, E.a, Maurano, F.a, Battistel, E.b, Parascandola, P.cd (literal)
Pagina inizio
  • 138 (literal)
Pagina fine
  • 142 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 81 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 2 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • a Dip. to Fisiol. Gen. ed Ambientale, Sezione Igiene e Microbiologia, Univ. Studi di Napoli Fedrico II, Via Mezzocannone 16, 80134 Napoli, Italy b ENICHEM S.p.a., Istituto Guido Donegani, Via G. Fauser 4, 29100 Novara, Italy c Dipto. Ingegneria Chim./Alimentare, Univ. degli Studi di Salerno, 84084 Fisdano (SA), Italy d Dip. to Fisiol. Gen. ed Ambientale, Sezione Igiene e Microbiologia, Univ. Studi di Napoli Federico II, Via Mezzocannone 16, 80134 Napoli, Italy (literal)
Titolo
  • D-amino acid oxidase from Trigonopsis variabilis: Immobilization of whole cells in natural polymeric gels for glutaryl-7-aminocephalosporanic acid production (literal)
Abstract
  • The enzymatic oxidation of cephalosporin C to glutaryl-7- aminocephalosporanic acid (glutaryl-7-ACA) was carried out utilizing permeabilized whole cells of the yeast Trigonopsis variabilis entrapped in Ca-alginate beads. The biomass, cultured in a rich medium containing DL- methionine and harvested after 72 h of growth, exhibited high levels of D- amino acid oxidase activity. Prior to use, the whole cells were permeabilized with four freeze-thawing cycles and immobilized in polysaccharide matrices, such as Ca-alginate and ?-carrageenan, and in an insolubilized gelatin gel. The best results in terms of activity yield and storage stability were obtained with cells entrapped in Ca-alginate beads. These cells were utilized for glutaryl-7-ACA production in a continuous stirred batch reactor (CSTR) and in a packed bed reactor working as a plug flow reactor (PFR), using 50 mM cephalosporin C as substrate. The performances of the two systems were compared. The overall productivities (calculated on a void volume basis) were 1.64 g and 255 mg of glutaryl-7-ACA l -1 h -1 in the PFR and in the CSTR, respectively. (literal)
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