http://www.cnr.it/ontology/cnr/individuo/prodotto/ID200021

Intravenous or intranasal administration of gliadin is able to down- regulate the specific immune response in mice (Articolo in rivista)

Type

Label

Intravenous or intranasal administration of gliadin is able to down- regulate the specific immune response in mice (Articolo in rivista) (literal)

Anno

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

Alternative label

Rossi, M.a, Maurano, F.a, Caputo, N.b, Auricchio, S.b, Sette, A.d, Capparelli, R.c, Troncone, R.b (1999)
Intravenous or intranasal administration of gliadin is able to down- regulate the specific immune response in mice
in Scandinavian journal of immunology (Print)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Rossi, M.a, Maurano, F.a, Caputo, N.b, Auricchio, S.b, Sette, A.d, Capparelli, R.c, Troncone, R.b (literal)

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Note

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a Inst. of Food Sci. and Technology, CNR, Avellino, Italy b Department of Pediatrics, University 'Federico II', Naples, Italy c School of Agriculture, University 'Federico II', Naples, Italy d Epimmune, San Diego, CA, United States (literal)

Titolo

Intravenous or intranasal administration of gliadin is able to down- regulate the specific immune response in mice (literal)

Abstract

The mucosal lesion in coeliac disease (CD) represents an immunologically mediated injury triggered by gliadin and is restricted by a particular assortment of major histocompatibility complex (MHC) class II genes. Therefore, immunomodulatory strategies to tolerize gliadin-specific, class II-restricted T-cell responses could represent an alternative to current treatments of CD, which are based on a gluten-free diet. In this study, BALB/c mice derived from a gluten-free diet colony were tolerized by either intranasal (i.n.) or intravenous (i.v.) administration of single or multiple doses of gliadin. While a single dose failed to induce tolerance, a significant decrease in gliadin-specific T-cell proliferation was detected (P < 0.001) after multiple i.n. or i.v. administrations. No significant difference in antibody titre was detected for antigen-specific immunoglobulin G (IgG) or the IgG1 subclass, but a lower IgG(2a)-specific titre was observed. Both interferon-? (IFN-?) and interleukin (IL)-2 expression, measured by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR), were reduced on antigen administration, both i.v. and i.n. Neither regimen showed a regulatory effect on IL-4 production. As T helper 1 (Th1) cytokines seem to be important in the pathogenesis of CD, our data therefore highlight the potential of i.n. and i.v. routes for the design of useful immunomodulatory strategies for CD. (literal)

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