http://www.cnr.it/ontology/cnr/individuo/prodotto/ID199970
Identification of glutathione-methacrylates adducts in gingival fibroblasts and erythrocytes by HPLC-MS and capillary electrophoresis (Articolo in rivista)
- Type
- Label
- Identification of glutathione-methacrylates adducts in gingival fibroblasts and erythrocytes by HPLC-MS and capillary electrophoresis (Articolo in rivista) (literal)
- Anno
- 2011-01-01T00:00:00+01:00 (literal)
- Alternative label
G. Nocca, R. Ragno, V. Carbone, G. E. Martorana, D. V. Rossetti, G. Gambarini, B. Giardina, A. Lupi (2011)
Identification of glutathione-methacrylates adducts in gingival fibroblasts and erythrocytes by HPLC-MS and capillary electrophoresis
in Dental materials
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- G. Nocca, R. Ragno, V. Carbone, G. E. Martorana, D. V. Rossetti, G. Gambarini, B. Giardina, A. Lupi (literal)
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- Institute of Biochemistry and Clinical Biochemistry, Catholic University, Rome, Italy
Dipartimento di Chimica e Tecnologie del Farmaco dell'Università La Sapienza, Rome, Italy
Istituto di Scienze dell' Alimentazione, CNR, Avellino, Italy
Department of Dental Science, Sapienza University of Rome, Rome, Italy
Istituto di Chimica del Riconoscimento Molecolare, CNR, Rome, Italy (literal)
- Titolo
- Identification of glutathione-methacrylates adducts in gingival fibroblasts and erythrocytes by HPLC-MS and capillary electrophoresis (literal)
- Abstract
- Objectives. Methacrylic monomers are released, from dental composite resins, either into the
oral cavity or in pulpal tissues, where they can cause local or systemic adverse effects. The
mechanisms of these effects are not well understood, probably because such molecules can
act at different levels also inducing a depletion of intracellular glutathione (GSH). GSH can
detoxify methacrylates by conjugating their ?,?-unsaturated carbon-carbon moiety to the
thiol group, with the catalysis of glutathione S-transferases (GST). This reaction determines
a GSH cellular depletion and belongs to the metabolism of ?,?-unsaturated esters, protecting
the body against the toxic effects of electrophiles. On the basis of the above considerations,
this work aim is to set up a method for the detection of the adducts formed by methacrylic
monomers with GSH in cells using HPLC coupled to mass spectrometry (HPLC-MS) and
micellar electrokinetic capillary chromatography (MECK) techniques.
Methods and results. Adducts of glutathione with triethylene glycol dimethacrylate (TEGDMA)
and hydroxyethyl methacrylate (HEMA) were incontrovertibly identified by HPLC-MS and
MECK in human gingival fibroblasts and erythrocytes, both outside and inside cells. Molecular
docking simulations ofHEMAand TEGDMA in the experimental structure of glutathione
S-transferase, are also reported to rationalize the effectiveness of such enzyme in the catalysis
of the above described reaction.
Significance. The setup of a method for the identification of GSH-methacrylate adducts allows
to determine when the metabolic pathway involving such compounds is employed by cells
for the detoxification of monomers leached from composite resins. (literal)
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