Electron microscope study on a transmissible cytotoxic factor isolated from cerebrospinal fluid of neurological patients: analysis of its structure and of its effects on various types of cell cultures (Articolo in rivista)

Type
Label
  • Electron microscope study on a transmissible cytotoxic factor isolated from cerebrospinal fluid of neurological patients: analysis of its structure and of its effects on various types of cell cultures (Articolo in rivista) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1136/jclinpath-2011-200034 (literal)
Alternative label
  • Guerra, D; Ronchetti, I; Beretti, F; Bartoletti, AM; Pietrosemoli, P; Gelormini, MS; Malagolini, N; Muscatello, U; Portolani, M (2012)
    Electron microscope study on a transmissible cytotoxic factor isolated from cerebrospinal fluid of neurological patients: analysis of its structure and of its effects on various types of cell cultures
    in Journal of Clinical Pathology
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Guerra, D; Ronchetti, I; Beretti, F; Bartoletti, AM; Pietrosemoli, P; Gelormini, MS; Malagolini, N; Muscatello, U; Portolani, M (literal)
Pagina inizio
  • 20 (literal)
Pagina fine
  • 28 (literal)
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  • 65 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Univ Modena & Reggio Emilia, Dept Lab Act Morbid Anat & Forens Med, I-41124 Modena, Italy Univ Modena & Reggio Emilia, Dept Biomed Sci, I-41124 Modena, Italy Univ Bologna, Dept Expt Pathol, I-40126 Bologna, Italy CNR, Inst Nanosci, Natl Res Ctr S3, Modena, Italy (literal)
Titolo
  • Electron microscope study on a transmissible cytotoxic factor isolated from cerebrospinal fluid of neurological patients: analysis of its structure and of its effects on various types of cell cultures (literal)
Abstract
  • Aims In previous studies, the present group showed that a factor, present in the cerebrospinal fluid of seven neuro-patients, was capable of inducing cell damages on cell cultures of epithelial cells (Vero), glial cells (DG54-MG) and human primary lymphocytes. The cytotoxicity, once induced, could be transmitted to fresh cell cultures using crude preparations obtained from the cytotoxic cell cultures. Methods and Results The present electron microscope study describes in detail the pathological changes occurring in the previously assayed cultured cell types, and for the first time in human fibroblasts, as a consequence of the treatment with crude cytotoxic preparation. It also demonstrates with reasonable certainty the absence of virus-like particles in both the treated cells and the partially purified cytotoxic fraction. Indeed high-resolution electron microscopy analysis shows that this fraction consists almost entirely of protein spheroids with a diameter of 8-12 nm. Conclusions The possible significance of nanotubular aggregates, observed in particular in the glial and in the epithelial cells undergoing cytotoxic damage, is also discussed. (literal)
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