Application of ultrafast gold luminescence to measuring the instrument response function for multispectral multiphoton fluorescence lifetime imaging (Articolo in rivista)

Type
Label
  • Application of ultrafast gold luminescence to measuring the instrument response function for multispectral multiphoton fluorescence lifetime imaging (Articolo in rivista) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1364/OE.19.013848 (literal)
Alternative label
  • Talbot CB, Patalay R, Munro I, Warren S, Ratto F, Matteini P, Pini R, Breunig HG, Konig K, Chu AC, Stamp GW, Neil MAA, French PMW, Dunsby C (2011)
    Application of ultrafast gold luminescence to measuring the instrument response function for multispectral multiphoton fluorescence lifetime imaging
    in Optics express; Optical Society of America, Washington (Stati Uniti d'America)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Talbot CB, Patalay R, Munro I, Warren S, Ratto F, Matteini P, Pini R, Breunig HG, Konig K, Chu AC, Stamp GW, Neil MAA, French PMW, Dunsby C (literal)
Pagina inizio
  • 13848 (literal)
Pagina fine
  • 13861 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 29 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
  • Scopu (literal)
  • PubMe (literal)
  • Google Scholar (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Univ London Imperial Coll Sci Technol & Med, Dept Phys, London SW7 2AZ, England Imperial Coll Healthcare NHS Trust, London W12 0HS, England CNR, Inst Fis Appl, I-50019 Sesto Fiorentino, Italy JenLab GmbH, D-66123 Saarbrucken, Germany Royal Marsden Hosp NHS Trust, London SW3 6JJ, England (literal)
Titolo
  • Application of ultrafast gold luminescence to measuring the instrument response function for multispectral multiphoton fluorescence lifetime imaging (literal)
Abstract
  • When performing multiphoton fluorescence lifetime imaging in multiple spectral emission channels, an instrument response function must be acquired in each channel if accurate measurements of complex fluorescence decays are to be performed. Although this can be achieved using the reference reconvolution technique, it is difficult to identify suitable fluorophores with a mono-exponential fluorescence decay across a broad emission spectrum. We present a solution to this problem by measuring the IRF using the ultrafast luminescence from gold nanorods. We show that ultrafast gold nanorod luminescence allows the IRF to be directly obtained in multiple spectral channels simultaneously across a wide spectral range. We validate this approach by presenting an analysis of multispectral autofluorescence FLIM data obtained from human skin ex vivo (literal)
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