Rapid resolution by reversed-phase high performance liquid chromatography of the thylakoid membrane proteins of the photosystem II light-harvesting complex (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Rapid resolution by reversed-phase high performance liquid chromatography of the thylakoid membrane proteins of the photosystem II light-harvesting complex (Articolo in rivista) (literal)

Anno

• 1997-01-01T00:00:00+01:00 (literal)

Alternative label

• Lello Zolla, Maria Bianchetti, Anna Maria Timperio, Danilo Corradini (1997)
  Rapid resolution by reversed-phase high performance liquid chromatography of the thylakoid membrane proteins of the photosystem II light-harvesting complex
  in Journal of chromatography (Print); Elsevier, Amsterdam (Paesi Bassi)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Lello Zolla, Maria Bianchetti, Anna Maria Timperio, Danilo Corradini (literal)

Pagina inizio

• 131 (literal)

Pagina fine

• 138 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 779 (literal)

Rivista

• Journal of chromatography (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 8 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 1-2 (literal)

Note

• ISI Web of Science (WOS), Scopus (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Dipartimento di Scienze Ambientali, Università della Tuscia, Viterbo; Istituto di Cromatografia del CNR, Montelibretti (Roma). (literal)

Titolo

• Rapid resolution by reversed-phase high performance liquid chromatography of the thylakoid membrane proteins of the photosystem II light-harvesting complex (literal)

Abstract

• This paper presents the results of a study performed to develop a rapid and straightforward HPLC method to resolve and identify the protein components of the photosystem II light-harvesting complex (LHC II), which is the major protein-chlorophyll a/b complex in higher plants. The method employs a reversed-phase Vydac Protein C4 column containing 5-?m porous butyl silica which is eluted by a 13.5-min linear water-acetonitrile gradient in the presence of 0.1% (v/v) trifluoroacetic acid. Peak identification was performed by denaturing SDS-PAGE analysis of the fractions of the eluate collected throughout the chromatographic run. This procedure allowed the identification of Lhcb2, Lhcb1 and Lhcb3 protein components of LHC II, which eluted as well-resolved peaks with highly repeatable retention times, in samples isolated from spinach leaves both by the same and by different preparations. The reversed-phase HPLC method has proven to be suitable for detecting dissimilarities in the protein components of LHC II isolated from different plants. (literal)

Editore

• Elsevier (Editore)

Prodotto di

• Istituto di metodologie chimiche (IMC) (Istituto)

Autore CNR

• DANILO CORRADINI (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Rapid resolution by reversed-phase high performance liquid chromatography of the thylakoid membrane proteins of the photosystem II light-harvesting complex" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• DANILO CORRADINI (Unità di personale interno)

Prodotto

• Istituto di metodologie chimiche (IMC) (Istituto)
• http://www.cnr.it/ontology/cnr/individuo/modulo/ID6875

Editore di

• Elsevier (Editore)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of chromatography (Rivista)

Insieme di parole chiave di

• Parole chiave di "Rapid resolution by reversed-phase high performance liquid chromatography of the thylakoid membrane proteins of the photosystem II light-harvesting complex" (Insieme di parole chiave)