Insights into subtle conformational differences in the substrate-binding loop of fungal 17beta-hydroxysteroid dehydrogenase: a combined structural and kinetic approach (Articolo in rivista)

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  • Insights into subtle conformational differences in the substrate-binding loop of fungal 17beta-hydroxysteroid dehydrogenase: a combined structural and kinetic approach (Articolo in rivista) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Alternative label
  • Cassetta A., Krastanova I., Kristan K., Brunskole ŠVegelj M., Lamba D., Lanisšnik Rižner T., Stojan J. (2012)
    Insights into subtle conformational differences in the substrate-binding loop of fungal 17beta-hydroxysteroid dehydrogenase: a combined structural and kinetic approach
    in Biochemical journal (Online)
    (literal)
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  • Cassetta A., Krastanova I., Kristan K., Brunskole ŠVegelj M., Lamba D., Lanisšnik Rižner T., Stojan J. (literal)
Pagina inizio
  • 151 (literal)
Pagina fine
  • 160 (literal)
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  • 441 (literal)
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  • doi:10.1042/BJ20110567 (literal)
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  • 10 (literal)
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  • 1 (literal)
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  • PubMe (literal)
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  • Cassetta A., IC-CNR, Trieste, Italy; Krastanova I., Sincrotrone Trieste, SCpA, Trieste, Italy; Kristan K., Institute of Biochemistry, Faculty of Medicine, UNiv. Ljubljiana, Slovenia; Brunskole ŠVegelj M., Institute of Biochemistry, Faculty of Medicine, UNiv. Ljubljiana, Slovenia; Lamba D., IC-CNR, Trieste, Italy; Lanisšnik Rižner T., Institute of Biochemistry, Faculty of Medicine, UNiv. Ljubljiana, Slovenia; Stojan J., Institute of Biochemistry, Faculty of Medicine, UNiv. Ljubljiana, Slovenia (literal)
Titolo
  • Insights into subtle conformational differences in the substrate-binding loop of fungal 17beta-hydroxysteroid dehydrogenase: a combined structural and kinetic approach (literal)
Abstract
  • The 17beta-HSD (17beta-hydroxysteroid dehydrogenase) from the filamentous fungus Cochliobolus lunatus (17beta-HSDcl) is a NADP(H)-dependent enzyme that preferentially catalyses the interconversion of inactive 17-oxo-steroids and their active 17beta-hydroxy counterparts. 17beta-HSDcl belongs to the SDR (short-chain dehydrogenase/reductase) superfamily. It is currently the only fungal 17beta-HSD member that has been described and represents one of the model enzymes of the cP1 classical subfamily of NADPH-dependent SDR enzymes. A thorough crystallographic analysis has been performed to better understand the structural aspects of this subfamily and provide insights into the evolution of the HSD enzymes. The crystal structures of the 17?-HSDcl apo, holo and coumestrol-inhibited ternary complex, and the active-site Y167F mutant reveal subtle conformational differences in the substrate-binding loop that probably modulate the catalytic activity of 17?-HSDcl. Coumestrol, a plant-derived non-steroidal compound with oestrogenic activity, inhibits 17beta-HSDcl [IC50 2.8 microM; at 100 microM substrate (4-oestrene-3,17-dione)] by occupying the putative steroid-binding site. In addition to an extensive hydrogen-bonding network, coumestrol binding is stabilized further by pi-pi stacking interactions with Tyr212. A stopped-flow kinetic experiment clearly showed the coenzyme dissociation as the slowest step of the reaction and, in addition to the low steroid solubility, it prevents the accumulation of enzyme-coenzyme-steroid ternary complexes. (literal)
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